TITLE

Disruption of the uptake hydrogenase gene, but not of the bidirectional hydrogenase gene, leads to enhanced photobiological hydrogen production by the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120

AUTHOR(S)

Masukawa, H.; Mochimaru, M.; Sakurai, H.

PUB. DATE

April 2002

SOURCE

Applied Microbiology & Biotechnology;Apr2002, Vol. 58 Issue 5, p618

SOURCE TYPE

Academic Journal

DOC. TYPE

Article

ABSTRACT

In order to determine the effects of the deletion of hydrogenase genes on nitrogenase-based photobiological H2 productivity by heterocystous N2-fixing cyanobacteria, we have constructed three hydrogenase mutants from Anabaena sp. PCC 7120: hupL� (deficient in the uptake hydrogenase), hoxH� (deficient in the bidirectional hydrogenase), and hupL�/hoxH� (deficient in both genes). The hupL� mutant produced H2 at a rate four to seven times that of the wild-type under optimal conditions. The hoxH� mutant produced significantly lower amounts of H2 and had slightly lower nitrogenase activity than wild-type. H2 production by the hupL�/hoxH� mutant was slightly lower than, but almost equal to, that of the hupL� mutant. The efficiency of light energy conversion to H2 by the hupL� mutant at its highest H2 production stage was 1.2% at an actinic visible light intensity of 10 W/m2 (PAR) under argon atmosphere. These results indicate that deletion of the hupL gene could be employed as a source for further improvement of H2 production in a nitrogenase-based photobiological H2 production system.

ACCESSION #

15681121

Tags: HYDROGENASE;  PHOTOBIOLOGY;  HYDROGEN;  ANABAENA;  CYANOBACTERIA;  ARGON;  NITROGENASE

 

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