Enhanced production of penicillin G acylase from a recombinant Escherichia coli

Vohra, Pawan Kumar; Sharma, Rakesh; Raj Kashyap, Des; Tewari, Rupinder
April 2001
Biotechnology Letters;Apr2001, Vol. 23 Issue 7, p531
Academic Journal
Penicillin G acylase (pac) gene was cloned into a stable asd+ vector (pYA292) and expressed in Escherichia coli. This recombinant strain produced 1000 units penicillin G acylase g-1 cell dry wt, which is 23-fold more than that produced by parental Escherichia coli ATCC11105. This enzyme was purified to 16 units mg-1 protein by a novel two-step process.


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