TITLE

Expression of the Cloned Hemagglutinin—Neuraminidase Gene of the Newcastle Disease Virus in Mouse Myeloma Cells

AUTHOR(S)
Risinskaya, N. V.; Fegeding, K. V.; Vasilenko, O. V.; Sudarikov, A. B.
PUB. DATE
September 2001
SOURCE
Molecular Biology;Sep2001, Vol. 35 Issue 5, p785
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Vaccination with autologous cancer cells expressing a potent foreign antigen is promising for immunotherapy of tumors. A construct was obtained to transfect cancer cells with the hemagglutinin–neuraminidase (HN) gene of the Newcastle disease virus (NDV). Specific primers were designed, and the HN cDNA was amplified from RNA isolated from the allantoic fluid of NDV-infected embryonated chicken eggs. The amplified fragment was cloned in pCR2.1, sequenced, and recloned in expression vector pCDNA3.1/Zeo(+). The resulting construct was used to transfect mouse myeloma cells SP2/0. Production of HN was checked by ELISA and by a neuraminidase activity assay. Cell agglutination on ice was proposed as a test for surface HN.
ACCESSION #
15605053

 

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