Characterisation of IE and UL5 gene products of equine herpesvirus 1 using DNA inoculation of mice

Koen, M. T.; Walker, C.; Wellington, J. E.; Love, D. N.; Whalley, J. M.
December 2000
Archives of Virology;Dec2000, Vol. 145 Issue 12, p2677
Academic Journal
Summary. The equine herpesvirus 1 (EHV-1) strain HVS25A regulatory genes IE and UL5, encoding homologues of herpes simplex virus 1 (HSV-1) ICP4 and ICP27 respectively, were cloned into a eukaryotic expression vector and the DNA injected intramuscularly into mice. Antibodies produced in this way detected the IE or UL5 gene products as diffuse material in nuclei of RK13 cells transfected with the individual genes but as discrete punctate or large aggregates in RK13 cells infected with EHV-1. Western blotting on EHV-1 infected RK13 cells showed multiple IE products of 120–200 kDa and a UL5 product of 52 kDa. Inoculation with plasmids expressing EHV-1 IE or UL5 provided limited protection against EHV-1 challenge in mice as determined by increased virus clearance from lungs on day 2 post-challenge and a reduction in severity of lung histopathology. However, this protection was relatively weak compared with that provided by inoculation of DNA encoding EHV-1 glycoprotein D (gD), possibly reflecting the importance of neutralising antibody in this model.


Related Articles

  • An Intravenous Stimulus Package for Oncolytic Virotherapy. Vile, Richard; Melcher, Alan // Molecular Therapy;Nov2011, Vol. 19 Issue 11, p1930 

    The article presents information related to oncolytic virotherapy (OV). Explanation regarding the development of oncolytic virus is presented. It is stated that within tumor tissue viral gene expression exists surrounded by very low levels of expression in normal tissue. It is mentioned that the...

  • Extending the transposable payload limit of Sleeping Beauty (SB) using the Herpes Simplex Virus (HSV)/SB amplicon-vector platform. de Silva, S.; Mastrangelo, M. A.; Lotta, Jr., L. T.; Burris, C. A.; Federoff, H. J.; Bowers, W. J. // Gene Therapy;Mar2010, Vol. 17 Issue 3, p424 

    The ability of a viral vector to safely deliver and stably integrate large transgene units (transgenons), which not only include one or several therapeutic genes, but also requisite native transcriptional regulatory elements, would be of significant benefit for diseases presently refractory to...

  • Identification of herpes simplex virus type 1 proteins encoded within the first 1.5 kb of the latency-associated transcript. Henderson, Gail; Jaber, Tareq; Carpenter, Dale; Wechsler, Steven L.; Jones, Clinton // Journal of NeuroVirology;Oct-Dec2009, Vol. 15 Issue 5/6, p439 

    Expression of the first 1.5 kb of the latency-associated transcript (LAT) that is encoded by herpes simplex virus type 1 (HSV-1) is sufficient for wild-type (wt) levels of reactivation from latency in small animal models. Peptide-specific immunoglobulin G (IgG) was generated against open reading...

  • Rising incidence and prevalence of herpes simplex type 2 infection in a cohort of 26 year old New Zealanders. Eberhart-Phillips, Jason E; Dickson, Nigel P; Paul, Charlotte; Herbison, G Peter; Taylor, Janette; Cunningham, Anthony L // Sexually Transmitted Infections;Oct2001, Vol. 77 Issue 5, p353 

    Objectives: To examine changes in the incidence and prevalence of herpes simplex type 2 (HSV-2) infection in a birth cohort of 26 year old New Zealanders in whom seroprevalence had been measured at 3.4% at age 21. Methods: Sen from 869 cohort members were tested using an indirect IgG enzyme...

  • Mollaret's Meningitis In Association With Herpes Simplex Virus Type 2. Sethi, Nitin K.; Favate, Albert; Karter, Dennis // Internet Journal of Infectious Diseases;2005, Vol. 4 Issue 2, p9 

    Benign recurrent aseptic meningitis, also called Mollaret's meningitis, is a rare, albeit well described disease. The exact etiology is unknown though Herpes Simplex Virus is now thought to be the most likely pathogen. We report a case of a 41-year old woman who experienced three episodes of...

  • Genome-Wide Screen of Three Herpesviruses for Protein Subcellular Localization and Alteration of PML Nuclear Bodies. Salsman, Jayme; Zimmerman, Nicole; Tricia Chen; Domagala, Megan; Frappier, Lori // PLoS Pathogens;Jul2008, Vol. 4 Issue 7, p1 

    Herpesviruses are large, ubiquitous DNA viruses with complex host interactions, yet many of the proteins encoded by these viruses have not been functionally characterized. As a first step in functional characterization, we determined the subcellular localization of 234 epitope-tagged proteins...

  • Esophageal achalasia: Is the herpes simplex virus really innocent? Castagliuolo, Ignazio; Brun, Paola; Costantini, Mario; Rizzetto, Christian; Palù, Giorgio; Costantino, Michela; Baldan, Nicola; Zaninotto, Giovanni; Palù, Giorgio // Journal of Gastrointestinal Surgery;Jan2004, Vol. 8 Issue 1, p24 

    This study was designed to test the hypothesis that mononuclear cells in the myenteric plexus of patients with achalasia may be activated by herpes simplex virus type 1 (HSV-1). Strips of esophageal muscle were obtained from patients with achalasia and multiorgan transplant donors who served as...

  • Expression, purification of herpes simplex virus type 1 US11 Protein, and production of US11 polyclonal antibody.  // Virology Journal;2011, Vol. 8 Issue 1, p490 

    The article focuses on the research presented by Yizhong Huang and colleagues on the study of US11 protein of herpes simplex virus type 1 (HSV-1) and the production of polyclonal antibody. It mentions that the US11 gene was combined into prokaryotic expression vector pET-32a (+) to provide...

  • Gadd34 functional domains involved in growth suppression and apoptosis. Christine Hollander, M; Poola-Kella, Silpa; Fornace, Albert J // Oncogene;6/19/2003, Vol. 22 Issue 25, p3827 

    Gadd34 (also known as MyD116) was originally described as a growth arrest and DNA damage-inducible gene. Increased expression of Gadd34 was subsequently found to correlate with apoptosis, and forced overexpression of the protein leads to apoptosis. Gadd34 protein modulates protein phosphatase...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics