Micro-Determination of Protein Containing SH- and -S-S- Groups Based on the Polarographic wave of an Electroactive Derivative

Jingui Lan; Dengbai Luo; Yanlan Feng
December 2004
Microchimica Acta;Dec2004, Vol. 148 Issue 3/4, p299
Academic Journal
This paper presents a new simple and sensitive method for the micro-determination of protein containing SH- and -S-S- groups based on the single sweep polarographic wave of an electroactive derivative. In 0.04?mol?L-1 Na3PO4 and 0.2% ascorbic acid solution, protein is heated in a boiling water bath for 15?min, the reaction product giving a sensitive reduction wave at -0.70?V (vs. SCE). The wave height is linearly proportional to the concentration of protein. The calibration curves of bovine serum albumin (BSA), human serum albumin (HSA), ovalbumin (OVA) and lysozyme (Lyso) are constructed under the optimal conditions. For BSA and HSA, the linear ranges and detection limits are 0.05-24?mg?L-1 and 0.02?mg?L-1, respectively. The method has been applied to the determination of protein in human serum samples with satisfactory results. The mechanism of the polarographic wave was also studied, and the results show that S2- ion is released from the protein molecule during the derivatization reaction, the wave being attributed to the reduction of HgS.


Related Articles

  • Performance, Egg Quality Traits, and Serum Metabolite Concentrations of Laying Hens Affected by Dietary Supplemental Chromium Picolinate and Vitamin C Under a Heat-Stress Condition. Torki, Mehran; Zangeneh, Samira; Habibian, Mahmood // Biological Trace Element Research;Feb2014, Vol. 157 Issue 2, p120 

    A 3 × 2 factorial experiment consisting three levels (0, 200, and 400 μg/kg) of chromium (chromium picolinate) and two levels (0 and 250 mg/kg) of vitamin C was employed to evaluate the effects of these dietary supplements on performance, egg quality traits, and serum biochemical...

  • HSA.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1096 

    An encyclopedia entry for the medical acronym "HSA," which stands for human serum albumin, is presented.

  • Blood from Grains. Breindl, Anette // BioWorld Today;11/7/2011, Vol. 22 Issue 216, Special section p2 

    The article reports that scientists from Wuhan University in China have produced recombinant human serum albumin from rice.

  • Synchronous Fluorescence Determination of Protein with Functional Organic Nanoparticles. Tingting Xia; Lun Wang; Guirong Bian; Ling Dong; Shi Hong // Microchimica Acta;Jun2006, Vol. 154 Issue 3/4, p309 

    A new method for detecting protein using a synchronous fluorescence scan technique with a novel composite organic nanoparticle has been developed. The novel functional organic nanoparticle, dodecyl benzene sulfonic acid sodium salt (DBSS)-capped nano-anthracene, is easily prepared by...

  • Assessment of hypoproteinaemic oedema: a simple physical sign. Henry, J.A.; Altmann, P. // British Medical Journal;4/8/1978, Vol. 1 Issue 6117, p890 

    Assessment of hypoproteinaemic oedema: a simple physical sign It is well known that hypoproteinaemia can cause pitting oedema. We have found that hypoproteinaemic oedema pits and recovers differently from other types of oedema but can find no reference to this in reports or texts on physical...

  • Bromophenol Blue Binding as a Probe to Study Urea and Guanidine Hydrochloride Denaturation of Bovine Serum Albumin. Halim, Adyani Azizah Abd.; Kadir, Habsah Abdul; Tayyab, Saad // Journal of Biochemistry;Jul2008, Vol. 144 Issue 1, p33 

    Urea and guanidine hydrochloride (GdnHCl) denaturation of bovine serum albumin (BSA) were investigated using bromophenol blue (BPB) binding as a probe. Addition of BPB to BSA produced an absorption difference spectrum in the wavelength range, 525–675 nm with a minimum at 587 nm and a...

  • Influences of haemodialysis on the binding sites of human serum albumin: possibility of an efficacious administration plan using binding inhibition. Toyotaka Nishio; Norito Takamura; Ryuuichi Nishii; Jin Tokunaga; Mitsuyoshi Yoshimoto; Keiichi Kawai // Nephrology Dialysis Transplantation;Jul2008, Vol. 23 Issue 7, p2304 

    Background. We have studied the possibility that low-dose treatment utilizing the inhibition that may occur between two drugs at the same site of human serum albumin (HSA) improves the pharmacological effects. The purpose is to elucidate the differences in the binding capacities of sites I and...

  • Synchronous fluorescence determination and molecular modeling of 5-Aminosalicylic acid (5-ASA) interacted with human serum albumin. Feng-ling Cui; Li-xia Qin; Fang Li; Hong-xia Luo // Journal of Molecular Modeling;Dec2008, Vol. 14 Issue 12, p1111 

    Abstract  In this paper, we proposed a new method for the determination of either human serum albumin (HSA) or 5-Aminosalicylic acid (5-ASA) by synchronous fluorescence spectra and examined the interaction between them using the molecular modeling method under simulative...

  • Effect of TCN2 776C>G on vitamin B12 cellular availability in end-stage renal disease patients. Födinger, Manuela; Veitl, Mario; Skoupy, Sonja; Wojcik, Jadwiga; Röhrer, Claudia; Hagen, Wolfgang; Puttinger, Heidi; Hauser, Anna-Christine; Vychytil, Andreas; Sunder-Plassmann, Gere // Kidney International;Sep2003, Vol. 64 Issue 3, p1095 

    Effect of TCN2 776C>G on vitamin B12 cellular availability in end-stage renal disease patients. Background. Transcobalamin II is a serum protein that transports vitamin B12 from the intestine to the tissues. This complex, holo-transcobalamin II, may reflect vitamin B12 availability in the body....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics