TITLE

The role of modifications in codon discrimination by tRNALysUUU

AUTHOR(S)
Murphy IV, Frank V.; Ramakrishnan, Venki; Malkiewicz, Andrzej; Agris, Paul F.
PUB. DATE
December 2004
SOURCE
Nature Structural & Molecular Biology;Dec2004, Vol. 11 Issue 12, p1186
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNALysUUU requires the modification N6-threonylcarbamoyladenosine at position 37 (t6A37), adjacent and 3'to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t6A37 and either 5-methylaminomethyluridine or 2-thiouridine at the wobble position (mnm5U34 or s2U34). Here we report crystal structures of modified tRNA anticodon stem-loops bound to the 30S ribosomal subunit with lysine codons in the A site. These structures allow the rationalization of how modifications in the anticodon loop enable decoding of both lysine codons AAA and AAG.
ACCESSION #
15243154

 

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