TITLE

Determination of thiol compounds in rat striatum microdialysate by HPLC with a nanosized CoHCF-modified electrode

AUTHOR(S)
Liu, Meichuan; Li, Ping; Cheng, Yuxiao; Xian, Yuezhong; Zhang, Chenglin; Jin, Litong
PUB. DATE
November 2004
SOURCE
Analytical & Bioanalytical Chemistry;Nov2004, Vol. 380 Issue 5/6, p742
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A cobalt hexacyanoferrate (CoHCF) nanoparticle (size ca. 60 nm) chemically modified electrode (CME) was fabricated and the electrochemical behavior of thiols at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon (GC) electrode and with a general CoHCF CME which was electrodeposited in the traditional manner, the present nanosized CoHCF CME efficiently performed electrocatalytic oxidation for glutathione (GSH) andL-Cysteine (L-Cys) with relatively high sensitivity, outstanding stability, and long-life. Combined with high-performance liquid chromatography (HPLC), the nanosized CoHCF CME was used for electrochemical determination (ECD) of GSH andL-Cys. The peak currents were a linear function of concentrations in the range 2.0×10-7 to 2.0×10-4 mol L-1 for both GSH andL-Cys, with detection limits of 1.2×10-7 and 1.0×10-7 mol L-1, respectively. Coupled with microdialysis sampling, the HPLC-ECD system has been successfully used to assess the GSH andL-Cys content of rat striatum.
ACCESSION #
15205848

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics