On-site manipulation of single whole-genome DNA molecules using optical tweezers

Oana, Hidehiro; Kubo, Koji; Yoshikawa, Kenichi; Atomi, Haruyuki; Imanaka, Tadayuki
November 2004
Applied Physics Letters;11/22/2004, Vol. 85 Issue 21, p5090
Academic Journal
In this letter, we describe a noninvasive methodology for manipulating single Mb-size whole-genome DNA molecules. Cells were subjected to osmotic shock and the genome DNA released from the burst cells was transferred to a region of higher salt concentration using optical tweezers. The transferred genome DNA exhibits a conformational transition from a compact state into an elongated state, accompanied by the change in its environment. The applicability of optical tweezers to the on-site manipulation of giant genome DNA is suggested, i.e., lab-on-a-plate.


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