Continuous microfluidic immunomagnetic cell separation

Inglis, David W.; Riehn, R.; Austin, R. H.; Sturm, J. C.
November 2004
Applied Physics Letters;11/22/2004, Vol. 85 Issue 21, p5093
Academic Journal
We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.


Related Articles

  • The use of morphomutants to investigate septum formation and cell separation in Schizosaccharomyces pombe. Sipiczki, Matthias; Bozsik, Aniko // Archives of Microbiology;Dec2000, Vol. 174 Issue 6, p386 

    Cytokinesis in the fission yeast Schizosaccharomyces begins by formation of a medially placed actomyosin ring, continues by progressive development of a septum, and is completed by cleavage of the mature septum to bring about cell separation. The cytological analysis of the Schizosaccharomyces...

  • Mathematical model of peripheral blood stem cell harvest kinetics. Mayer, J; Pospíšil, Z; Korístek, Z // Bone Marrow Transplantation;Oct2003, Vol. 32 Issue 8, p749 

    Summary:A mathematical model of peripheral blood stem cell harvests was developed, taking two new parameters R (number of recruited cells/minute) and Ef (efficiency of collection) into consideration in addition to concentrations and collected amounts of cells. This model was tested on 241...

  • Advantage of combining magnetic cell separation with sperm preparation techniques. Said, Tarner M.; Grunewald, Sonja; Paasch, Uwe; Glander, Hans-Juergen; Baumann, Thomas; Kriegel, Christian; Liang Li; Agarwal, Ashok // Reproductive BioMedicine Online (Reproductive Healthcare Limited;Jun2005, Vol. 10 Issue 6, p740 

    The selection of vital, non-apoptotic spermatozoa is a prerequisite for achieving optimal conception rates in assisted reproductive techniques. Magnetic cell sorting using annexin-V microbeads can effectively separate apoptotic and non-apoptotic spermatozoa. The objective of the present study...

  • © PANCREATIN-EDTA TREATMENT AFFECTS BUOYANCY OF CELLS IN COHN FRACTION V PROTEIN DENSITY GRADIENTS WITHOUT RESIDUAL EFFECT ON CELL SIZE. Sheridan, J.W.; Simmons, R.J. // Australian Journal of Experimental Biology & Medical Science;Dec1983, Vol. 61 Issue 6, p727 

    Reports on the finding that the buoyancy of suspension-grown Mastocytoma P815 X-2 cells in albumin-rich Cohn fraction V (CFVP) density gradients is affected by prior incubation of the cells in pancreatin-EDTA salt solution. Observation that pancreatin-EDTA treatment had no effect on the buoyancy...

  • Purification and unique properties of mammary epithelial stem cells. Stingl, John; Eirew, Peter; Ricketson, Ian; Shackleton, Mark; Vaillant, François; Choi, David; Li, Haiyan I.; Eaves, Connie J. // Nature;2/23/2006, Vol. 439 Issue 7079, p993 

    Elucidation of the cellular and molecular mechanisms that maintain mammary epithelial tissue integrity is of broad interest and paramount to the design of more effective treatments for breast cancer. Evidence from both in vitro and in vivo experiments suggests that mammary cell differentiation...

  • Printing nanoparticle building blocks from the gas phase using nanoxerography. Barry, Chad R.; Lwin, Nyein Z.; Wei Zheng; Jacobs, Heiko O. // Applied Physics Letters;12/29/2003, Vol. 83 Issue 26, p5527 

    This letter reports on the electrostatic driven self-assembly of nanoparticles onto charged surface areas (receptors) from the gas phase for nanoparticle based device fabrication. The charged areas were generated by a parallel technique that uses a flexible, conductive electrode to pattern...

  • Micromachined cascade virtual impactor with a flow rate distributor for wide range airborne particle classification. Kim, Yong-Ho; Maeng, Jwa-Young; Park, Dongho; Jung, Il-Hyun; Hwang, Jungho; Kim, Yong-Jun // Applied Physics Letters;7/23/2007, Vol. 91 Issue 4, p043512 

    This letter reports a module for airborne particle classification, which consists of a micromachined three-stage virtual impactor for classifying airborne particles according to their size and a flow rate distributor for supplying the required flow rate to the virtual impactor. Dioctyl sebacate...

  • Amplification of spin-current polarization. Saha, D.; Holub, M.; Bhattacharya, P. // Applied Physics Letters;8/13/2007, Vol. 91 Issue 7, p072513 

    A ferromagnet/semiconductor based electrically controlled spin-current amplifier using a dual-drain nonlocal lateral spin valve is demonstrated. The spin polarization injected by the source into the channel is amplified at the second drain contact. An amplified current spin polarization of 100%...

  • Reply to Comment on¶“On the relationship between the entropy and enthalpy of interfacial segregation” by P. Lejček and S. Hofmann. Rubinovich, L.; Polak, M. // European Physical Journal B -- Condensed Matter;Feb2003, Vol. 31 Issue 3, p443 

    : Eur. Phys. J. B 22, 267 (2001) This note aims to resolve some terminological misunderstanding reflected by the comment of Lejček and Hofmann (L-H). It stems primarily from the difference in two reference systems quite commonly used in the description of interfacial segregation. Our article...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics