Freezing curve-based monitoring to quickly evaluate the viability of biological materials subject to freezing or thermal injury

Jing Liu; Yi-Xin Zhou
September 2003
Analytical & Bioanalytical Chemistry;Sep2003, Vol. 377 Issue 1, p173
Academic Journal
This paper is aimed at investigating the roles of freezing dynamics of a liquid droplet to characterize the properties of the material. In particular, freezing curve-based monitoring was proposed to quickly evaluate the viability of biological materials subject to freezing, re-warming, or other kinds of injury, which is an extremely important issue in practices such as cryobiology, hyperthermia, or freshness evaluation of bio-samples. An integrated micro analysis device was fabricated which is simple in structure and cheap to make. Preliminary freezing results demonstrated that minor changes in a biological material due to freezing or warming injury might result in a significant deviation of its freezing curve from that of the intact biomaterials. Several potential thermal indexes to quantify the material features were pointed out. Further, experiments were performed on some freezing and thawing processes of small amount of water on a cooling surface to test the effects of droplet sizes, measurement sites, cooling strength, and cooling geometry, etc., on the freezing responses of a water droplet. Their implementation in developing a new micro analysis system were suggested. This freezing curve-based monitoring method may open a new strategy for the evaluation of biomaterials subject to destruction in diverse fields.


Related Articles

  • Analysis of the Warming of Frozen Soil around a Cooling Column after Its Disconnection. Khrustalev, L. N. // Soil Mechanics & Foundation Engineering;Mar2001, Vol. 38 Issue 2, p69 

    A computational procedure, which takes into account phase transformations of the ice-water in a mass of precooled (frozen) soil after disconnection of the cooling system, is proposed; this makes it possible to predict the bearing capacity of the mass more accurately.

  • 5 Steps to Prevent Fluid Freezing. Mohapatra, Satish C. // Process Cooling & Equipment;Jul/Aug2004, p22 

    Discusses steps to prevent fluid freezing. Avoiding of system shutdown; Choice of fluid; Testing of fluid at regular intervals; Use of inert gas in the reservoir tank. INSET: Melting Point Is Key.


    Chapter 40 of the book "2006 American Society of Heating, Refrigeration and Air-Conditioning Engineers Handbook (ASHRAE)-Refrigeration" is presented. It explores the principles which contributes towards the growth and development of low-temperature biological applications of cryogenic...

  • Prepare your home for colder weather.  // Wentzville Journal;1/11/2012, Vol. 50 Issue 2, pD3 

    The article offers suggestions to prepare the home and make sure it is comfortable and protected during the colder weather.

  • MODELLING SEA ICE GROWTH. McGuinness, Mark J. // ANZIAM Journal;2009, Vol. 50 Issue 3, p306 

    The freezing of water to ice is a classic problem in applied mathematics, involving the solution of a diffusion equation with a moving boundary. However, when the water is salty, the transport of salt rejected by ice introduces some interesting twists to the tale. A number of analytic models for...

  • Record temperatures set with atomic cooling. Hecht, Jeff // Laser Focus World;May95, Vol. 31 Issue 5, p36 

    Reports on experiments that used laser cooling to measure record-low temperatures. Cooling of cesium atoms at the National Institute of Standards and Technology in Gaithersburg, Maryland; Cooling of rubidium atoms at the Joint Institute for Laboratory Astrophysics in Boulder, Colorado; Physical...

  • cryopreservation.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p551 

    A definition of the medical term "cryopreservation," which refers to the preservation at very low temperatures of biological materials, is presented.

  • Low-temperature deposition of rutile film on biomaterials substrates and its ability to induce apatite deposition in vitro. Jin-Ming Wu; Jin-Fang Liu; Satoshi Hayakawa; Kanji Tsuru; Akiyoshi Osaka // Journal of Materials Science: Materials in Medicine;Aug2007, Vol. 18 Issue 8, p1529 

    Low-temperature deposition of crystalline titania films on intrinsically bioinert materials to induce the bioactivity is of practical interest, not only because it meets the demand of providing organic biomaterials with bioactivity, which cannot tolerate high-temperature thermal treatments, but...

  • The half-life of 198Au : High-precision measurement shows no temperature dependence. Goodwin, J. R.; Golovko, V. V.; Iacob, V. E.; Hardy, J. C. // European Physical Journal A -- Hadrons & Nuclei;Dec2007, Vol. 34 Issue 3, p271 

    We have measured the half-life of the β -decay of 198Au in a metallic environment, both at low temperature (19K), and also at room temperature. We find the half-lives at both temperatures to be the same within 0.04%, a finding that contradicts a recent report of a 3.6±1.0 % difference in...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics