Trace determination of peptides in water samples using packed capillary liquid chromatography with UV and MS detection and characterization of peptide oxidation products by MS

Bjellaas, Thomas; Holm, Anders; Molander, Paal; Tørnes, John Aasulf; Greibrokk, Tyge; Lundanes, Elsa
February 2004
Analytical & Bioanalytical Chemistry;Feb2004, Vol. 378 Issue 4, p1021
Academic Journal
A capillary liquid chromatographic column switching method has been developed for fast and sensitive determination of peptides in water samples. Sample volumes of 1 mL were loaded onto a (320 μm I.D. ×30 mm) 10 μm Kromasil C18 pre-column, providing on-line analyte enrichment, prior to back-flushed elution onto a (320 μm I.D. ×150 mm) 3.5 μm Kromasil C18 analytical column. Loading flow rates of 250 μL/min and a mobile phase composition of acetonitrile/water/trifluoroacetic acid (22/77.9/0.1, v/v) provided a total analysis time of less than 25 minutes for the test peptides angiotensin II, bombesin, bradykinin, corazonin, neurotensin and substance P, using temperature programmed elution. In addition, solvent gradient elution and combined solvent gradient elution and temperature programming were explored. Using on-capillary UV detection at 210 nm resulted in a concentration limit of detection (cLOD) of about 1 ng/mL. The method was validated over the concentration range 1–100 ng/mL, yielding a coefficient of correlation of 0.997 or better. The within-assay (n=6) and between-assay (n=6) precisions of peak areas were on average 6% RSD and 5% RSD, respectively. When the method was applied to spiked chlorinated tap water samples, it was found that peptides containing methionine, tryptophan and cystine were oxidized. Identification of the oxidation products of the peptides in hypochlorite-treated water was done with positive electrospray ionization time-of-flight mass spectrometric detection.


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