Application of l-cysteine-capped nano-ZnS as a fluorescence probe for the determination of proteins

Chang Qing Zhu; Dan Hua Zhao; Jin Long Chen; Yong Xin Li; Le Yu Wang; Lun Wang; Yun You Zhou; Shu Juan Zhuo; Yu Qin Wu
February 2004
Analytical & Bioanalytical Chemistry;Feb2004, Vol. 378 Issue 3, p811
Academic Journal
Nanometer-sized l-cysteine-capped ZnS particles have been synthesized and used as a fluorescence probe to investigate the effect of proteins on fluorescent intensity. With Δλ=190 nm, maximum and constant synchronous fluorescence enhancement was produced at 267 nm and pH 5.12 in the presence of proteins. A highly sensitive synchronous fluorescence method for the rapid determination of proteins has been developed. Under optimum conditions, calibration graphs are linear over the range 0.03–8.0 μg mL-1 for bovine serum albumin (BSA), 0.01–6.0 μg mL-1 for human serum albumin (HSA), 0.05–8.0 μg mL-1 for γ-globulin (γ-G), and 0.04–4.0 μg mL-1 for ovalbumin, respectively. The relative standard deviations of seven replicate measurements were 1.75% for 1.0 μg mL-1 BSA, 1.90% for 1.0 μg mL-1 HSA, 1.65% for 1.0 μg mL-1 γ-G, and 2.32% for 1.0 μg mL-1 ovalbumin.


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