Use of reporter cell lines for detection of endocrine-disrupter activity

Willemsen, Philippe; Scippo, Marie-Louise; Kausel, Gudrun; Figueroa, Jaime; Maghuin-Rogister, Guy; Martial, Joseph A.; Muller, Marc
February 2004
Analytical & Bioanalytical Chemistry;Feb2004, Vol. 378 Issue 3, p655
Academic Journal
We have studied stable transformed human mammary cell lines with highly inducible steroid receptor-mediated luciferase reporter gene expression. Cells responding specifically to glucocorticoids, progestagens, androgens, or estrogens are described and characterized. The use of this high-throughput, cell-based assay for analysis of steroid (ant)agonists is reported. Systematic characterization of endocrine-disrupting activity on human receptors and in a human-cell system is interpreted for a selection of xenobiotics. We show that the phytoestrogens apigenin and genistin have progestagenic and androgenic activity, respectively. Finally, application of cell-based assays to the analysis of environmental samples is discussed.


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