Immunosensor for estrone with an equal limit of detection as common analytical methods

Tschmelak, Jens; Proll, Guenther; Gauglitz, Guenter
February 2004
Analytical & Bioanalytical Chemistry;Feb2004, Vol. 378 Issue 3, p744
Academic Journal
Immunoanalytical methods at a very low limit of detection (LOD) and a low limit of quantification (LOQ) are becoming more and more important for environmental analysis and especially for monitoring drinking water quality. Biosensors have suitable characteristics such as efficiency in allowing very fast, sensitive, and cost-effective detection. Here we describe a fully automated immunoassay for estrone with a LOD below 0.20 ng L–1 and a LOQ below 1.40 ng L–1. In contrast to common analytical methods such as GC-MS or HPLC-MS, the biosensor used requires no sample pre-treatment and pre-concentration. The basis of our sensitive assay is the antibody with a high affinity constant towards estrone. The very low amount of antibody per sample results in low validation parameters (LOD, LOQ, and IC50), but this assay for estrone represents the current device-related limitation of the River Analyser (RIANA).


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