A rapid two-step chromatographic method for the quantitative determination of vitellogenin in fish plasma

Jing Shao; Guoqing Shi; Jingfu Liu; Guibin Jiang
February 2004
Analytical & Bioanalytical Chemistry;Feb2004, Vol. 378 Issue 3, p615
Academic Journal
By combining anion-exchange membrane purification with high-performance size-exclusion chromatography (HPSEC) analysis, a two-step chromatographic method was developed for the determination of vitellogenin (Vtg) in fish plasma. Most plasma protein interferences can be removed during anion-exchange membrane purification process. Vtg is eluted from the size-exclusion chromatography column with a retention time of about 9 min and is characterized based on the native molecular weight, with a limit of quantification of 20 μg Vtg mL-1 plasma. The spiked recovery and interassay variability were better than 80% and 4.8%. This method was successfully applied to analyze the plasma Vtg levels of loach (Misgurnus angaillicaudatus) and sea catfish (Enchelyopus elongatus). In addition to all the female fish, Vtg is detected in 75% of male loaches and 100% of male sea catfish. The result indicates that some chemicals or unknown factors with estrogenic activity have induced male fish to produce Vtg.


Related Articles

  • The effect of the presence of volatile organoselenium compounds on the determination of inorganic selenium by hydride generation. Moreno, M. Eva; P�rez-Conde, Concepci�n; C�mara, Carmen // Analytical & Bioanalytical Chemistry;Mar2003, Vol. 375 Issue 5, p666 

    As a result of microbiological activity it is possible to find dimethylselenium (DMSe) and dimethyldiselenium (DMDSe) in a wide type of environmental samples, such as soils, sediments, sewage sludges and plants where methylation can take place. Selenium determination by hydride-generation (HG)...

  • Chromatographic methods and techniques used in studies of coals, their progenitors and coal-derived materials. Zubkova, Valentina // Analytical & Bioanalytical Chemistry;May2011, Vol. 399 Issue 9, p3193 

    The use of chromatography in studies of coals, their progenitors and coal-related products was reviewed. The specificity of the coal structure was discussed. The use of extraction in preparing study samples was discussed paying special attention to the occurrence of undesirable phenomena such as...

  • Investigations in Improving the Separation of PEGylated Proteins Using Jupiter 300 C4. Knudson, Vita; Farcas, Tividar; McGinley, Michael // LC-GC North America;Jun2007 Application Notebook Suppl, Vol. 25, p16 

    The article presents a study which investigates the improved reverse phase chromatography of PEGylated proteins. It was noticed that reverse phase chromatography gave improved resolution pf PEGylated proteins and unmodified proteins versus gel filtration chromatography. Reversed phase...

  • Chiral recognition applications of molecularly imprinted polymers: a critical review. Maier, Norbert M.; Lindner, Wolfgang // Analytical & Bioanalytical Chemistry;Sep2007, Vol. 389 Issue 2, p377 

    Molecular imprinting technology offers the unique opportunity to tailor chiral stationary phases with predefined chiral recognition properties by employing the enantiomers of interest as binding-site-forming templates. Added advantages, such as ease of preparation, chemical robustness, low-cost...

  • 399. Confirmed: Subcutaneous less costly than intravenous. Besarab, A.; Reyes, C.M.; Hornberger, J. // Kidney;May/Jun2003, Vol. 12 Issue 3, p146 

    Objective: To summarize results of a metaanalysis of clinical studies comparing average weekly IV and SC epoetin doses to maintain target hematocrit or hemoglobin levels. The chugcost difference between administration routes was assessed. Methodology: A metaanalysis of comparative studies of...

  • Biochemical response of the African catfish: Clarias gariepinus (Burchell, 1822) to sublethal concentrations of potassium permanganate. Kori-Siakpere, O.; Ikomi, R. B.; Ogbe, M. G. // Annals of Biological Research;2011, Vol. 2 Issue 2, p1 

    Potassium permanganate (KMnO4) is a widely used freshwater aquaculture chemotherapeutant for the treatment and prevention of waterborne parasitic and fungal diseases. The aim of this research was to determine its effects on some biochemical parameters of the widely consumed African catfish,...

  • For integrity's sake, crop award winners subject to random testing. Smith, Ron // Southwest Farm Press;9/7/2006, Vol. 33 Issue 17, p4 

    The author reflects on the blood testing requirement that all finalists for the U.S. Farm Press cotton and peanut awards must undergo. The blood testing requirement for all finalist is being implemented to achieve certainty that its winners is free from any performance-enhancing substances....

  • Blood-Monitoring Chip.  // Futurist;May/Jun87, Vol. 21 Issue 3, p3 

    Describes the development and use a silicon blood-monitoring chip that detects ions in blood which may be used as a lifesaver during surgical operations. Methods for using the chip; Use of the chip for the visual analysis of the patient's blood; Development of the chip by doctors at Newcastle...

  • Blood Chemistry Analysis as a Nutritional Diagnostic Tool. Cima, James // American Chiropractor;Jul2005, Vol. 27 Issue 8, p34 

    Discusses the blood chemistry analysis as a tool for nutritional diagnostic. Use of blood testing diagnose chemical imbalance of the body; Duplication and reproduction properties of blood in the analysis; Determinant of the physical adjustment needed on patients.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics