TITLE

Optical photothermal detection in HPLC

AUTHOR(S)
Proskurnin, M. A.; Luk'yanov, A. Yu.; Bendrysheva, S. N.; Bendryshev, A. A.; Pirogov, A. V.; Shpigun, O. A.
PUB. DATE
April 2003
SOURCE
Analytical & Bioanalytical Chemistry;Apr2003, Vol. 375 Issue 8, p1204
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A mode-mismatched parallel dual-beam thermal lens spectrometer with a far-field single-channel detector system was used as a detector in HPLC. An expert estimation of the measurement results was applied to optimize the optical-scheme configuration of the spectrometer to achieve the longest linear calibration range and highest repeatability under chromatographic flow conditions. Chelates with 4-(2-pyridylazo)resorcinol were separated and determined with the limits of detection of n × 10-8–n × 10-7 mol L-1; the relative standard deviation of measurements was 46%. Xylenol Orange, 4-(2-thiazolylazo)resorcinol, and dithizone were studied as post-column reagents in thermal lens detection in ion chromatography. The limits of detection were n × 10-8–n × 10-7 mol L-1; the linear calibration ranges were about three orders; the relative standard deviation of measurements was 3–7%. A combined photothermal–refractometric detector for HPLC based on a polarization interferometer is proposed. Metal complexes as 4-(pyridylazo)resorcinol chelates (limits of detection of n × 10-8–n × 10-7 mol L-1) and sugars (limits of detection of 10–20 ng L-1) were investigated as model substances. Obtained results were compared with results for traditional detectors, which show that photothermal detection has higher sensitivity than photometric and other absorption detectors.
ACCESSION #
15125597

 

Related Articles

  • Surface states and buried interface states studies in semiconductors by photothermal deflection spectroscopy. Zammit, U.; Marinelli, M.; Pizzoferrato, R. // Journal of Applied Physics;3/1/1991, Vol. 69 Issue 5, p3286 

    Presents a method which, through the analysis of the photothermal deflection spectroscopy (PDS) signal amplitude and phase spectra, enables to detect surface states and buried interface states and to measure their absorption. Function of PDS; One dimensional geometry for a three layer sample;...

  • Simple color center laser optothermal molecular beam spectrometer for high-resolution infrared spectroscopy. Kyrö, E. K.; Shoja-Chaghervand, P.; Eliades, M.; Danzeiser, D.; Bevan, J. W. // Review of Scientific Instruments;Jan1986, Vol. 57 Issue 1, p1 

    A broadband tunable single-frequency color center laser optothermal molecular beam spectrometer has been constructed. K components have been resolved for P(18) to R(11) rovibrational transitions in ν1 CH3C≡C–H. The observed transition linewidths are demonstrated to be ≤6...

  • Confirmation of the metastability of HF (v=1) in helium nanodroplets. Lindsay, C. M.; Lewis, W. K.; Miller, R. E. // Journal of Chemical Physics;9/22/2004, Vol. 121 Issue 12, p6095 

    Infrared laser spectroscopy is reported for HF in helium nanodroplets. The purpose of the present study is to resolve a discrepancy in the literature, namely, between the previous optothermal study, which indicates the HF does not vibrationally relax in the droplets, with an earlier mass...

  • Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy. Sijia Lu; Wei Min; Shasha Chong; Holtom, Gary R.; Xie, X. Sunney // Applied Physics Letters;3/15/2010, Vol. 96 Issue 11, p113701 

    Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation...

  • ZnO films obtained by photoelectrothermal oxidation, their properties, and possibilities of practical application. Zagoruiko, Yu. A.; Kovalenko, N. O.; Mateychenko, P. V.; Fedorenko, O. A.; Fedorov, A. G. // Crystallography Reports;Dec2008, Vol. 53 Issue 7, p1173 

    Zinc oxide films of optical quality are obtained on single-crystal ZnSe substrates by the developed method of photoelectrothermal oxidation. Comparative analysis of the structure and mechanical and electrical characteristics of the films, obtained by the methods of photothermal and...

  • Standoff detection of explosive residues using photothermal microcantilevers. Van Neste, C. W.; Senesac, L. R.; Yi, D.; Thundat, T. // Applied Physics Letters;3/31/2008, Vol. 92 Issue 13, p134102 

    Standoff detection of trace explosives is gaining attention due to its immediate relevance in countering terrorist threats based on explosive devices. However, most currently available standoff techniques rely on expensive, complex, and bulky equipment. We have demonstrated highly selective and...

  • Spatially localized measurement of thermal conductivity using a hybrid photothermal technique. Hua, Zilong; Ban, Heng; Khafizov, Marat; Schley, Robert; Kennedy, Rory; Hurley, David H. // Journal of Applied Physics;May2012, Vol. 111 Issue 10, p103505 

    A photothermal technique capable of measuring thermal conductivity with micrometer lateral resolution is presented. This technique involves measuring separately the thermal diffusivity, D, and thermal effusivity, e, to extract the thermal conductivity, k = (e2/D)1/2. To generalize this approach,...

  • Iron--Chelating Agents in Non--Iron Overload Conditions. Voest, E.E.; Vreugdenhil, G.; Marx, J.J.M // Annals of Internal Medicine;3/15/94, Vol. 120 Issue 6, p490 

    Provides information on a study that reviewed clinical experience with iron chelators in non-iron overload conditions. Details on inhibition of free radical-mediated tissue damage; Antiproliferative effects; Miscellaneous applications; Toxic effects; Conclusion.

  • Pulsed photothermal temperature profiling of agar tissue phantoms. Milaniň, Matija; Majaron, Boris; Nelson, J. Stuart // Lasers in Medical Science;Dec2007, Vol. 22 Issue 4, p279 

    We determine experimentally the accuracy of pulsed photothermal radiometric (PPTR) temperature depth profiling in water-based samples. We use custom tissue phantoms composed of agar gel layers separated by very thin absorbing layers. Two configurations of the acquisition system are compared, one...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics