Optical photothermal detection in HPLC

Proskurnin, M. A.; Luk'yanov, A. Yu.; Bendrysheva, S. N.; Bendryshev, A. A.; Pirogov, A. V.; Shpigun, O. A.
April 2003
Analytical & Bioanalytical Chemistry;Apr2003, Vol. 375 Issue 8, p1204
Academic Journal
A mode-mismatched parallel dual-beam thermal lens spectrometer with a far-field single-channel detector system was used as a detector in HPLC. An expert estimation of the measurement results was applied to optimize the optical-scheme configuration of the spectrometer to achieve the longest linear calibration range and highest repeatability under chromatographic flow conditions. Chelates with 4-(2-pyridylazo)resorcinol were separated and determined with the limits of detection of n × 10-8–n × 10-7 mol L-1; the relative standard deviation of measurements was 46%. Xylenol Orange, 4-(2-thiazolylazo)resorcinol, and dithizone were studied as post-column reagents in thermal lens detection in ion chromatography. The limits of detection were n × 10-8–n × 10-7 mol L-1; the linear calibration ranges were about three orders; the relative standard deviation of measurements was 3–7%. A combined photothermal–refractometric detector for HPLC based on a polarization interferometer is proposed. Metal complexes as 4-(pyridylazo)resorcinol chelates (limits of detection of n × 10-8–n × 10-7 mol L-1) and sugars (limits of detection of 10–20 ng L-1) were investigated as model substances. Obtained results were compared with results for traditional detectors, which show that photothermal detection has higher sensitivity than photometric and other absorption detectors.


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