Comparative study of coulometric and amperometric detection for the determination of macrolides in human urine using high-performance liquid chromatography

de la Huebra, Maria-José González; Bordin, Guy; Rodríguez, Adela R.
April 2003
Analytical & Bioanalytical Chemistry;Apr2003, Vol. 375 Issue 8, p1031
Academic Journal
A high-performance liquid chromatography (HPLC) method using chromatographic conditions optimised in a previous work was applied for the separation of three macrolide antibiotics roxithromycin (Rox), oleandomycin (Ole) and rosamicin (Ros) and further determination of two of them, roxithromycin (Rox) and oleandomycin (Ole), in human urine samples. A comparative study of the behaviour of these macrolides under the two types of electrochemical detection (EC) widely coupled with HPLC, that is coulometric (EC–C) and amperometric (EC–A), was carried out by applying the same multiresidue method. From the assays performed using both detectors the comparison was made taking relevant criteria such as detection limits, linearity, recovery and precision values into account. As a result of this comparison, the coulometric detector appears slightly more suitable than the amperometric one for macrolide analysis.


Related Articles

  • Determination of roxythromycin in blood serum by liquid chromatography with mass spectrometric detection. Semak, I.; Alekseev, N.; Korik, E.; Drobyshevskii, A.; Antonova, M. // Journal of Analytical Chemistry;Feb2011, Vol. 66 Issue 2, p195 

    procedure has been developed for the determination of a macrolide antibiotic roxythromycin (RX) in blood serum using HPLC with mass spectrometric detection using clarithromycin (CL) as the internal standard. RX and CL have been extracted from the samples by solid-phase extraction in a cartridge...

  • Ultra Performance Liquid Chromatography (UPLC): An Introduction. Swartz, Michael E. // LC-GC North America;May2005 Supplement, Vol. 23, p8 

    The article presents information on Ultra Performance Liquid Chromatography (UPLC). High performance liquid chromatography (HPLC) is a proven technique that has been used in laboratories worldwide over the past 30-plus years. By using smaller particles, speed and peak capacity (number of peaks...

  • The Use of ACQUITY UPLC in Pharmaceutical Development. Jerkovich, Anton D.; LoBrutto, Rosario; Vivilecchia, Richard V. // LC-GC North America;May2005 Supplement, Vol. 23, p15 

    The article presents information on the use of ACQUITY UPLC in Pharmaceutical Development. The most straightforward and accepted way to improve performance and gain speed in HPLC columns has been to reduce the particle diameter of the packing material. The ACQUITY system is capable of pumping...

  • Column Pressure Considerations in Analytical HPLC. Majors, Ronald E. // LC-GC North America;Nov2007, Vol. 25 Issue 11, p1074 

    The article examines the role of pressure in high performance liquid chromatography (HPLC). It looks at the impact of ultrahigh pressures encountered in ultrahigh-pressure liquid (UHPLC) on chromatographic parameters. It also addresses the concern of pressure changes in column pressure dictated...

  • Adjustment to the Chromatographic System in US Pharmacopeia Monographs: Flexibility and Limitations. Ravichandran, Ravi; Pappa, Horacio N.; Majors, Ronald E. // LC-GC North America;Oct2009, Vol. 27 Issue 10, p880 

    To meet system suitability requirements, chromatographers might need to make adjustments to the high performance liquid chromatography (HPLC) operating conditions. Users are allowed to implement these adjustments to bring the system into suitable performance without a full validation of the...

  • A Global Approach to HPLC Column Selection Using Reversed-Phase and HILIC Modes: What To Try When C18 Doesn't Work. Bicking, Merlin K. L.; Henry, Richard A. // LC-GC North America;Mar2010, Vol. 28 Issue 3, p234 

    Experimental data are presented to help understand high performance liquid chromatography column retention in a way that should make column selection and method development proceed faster when initial results with (18 columns are disappointing. Three stationary phases having widely different...

  • Method Translation in Liquid Chromatography. Majors, Ronald E. // LC-GC North America;Jun2011, Vol. 29 Issue 6, p476 

    The article focuses on the use of method translation in liquid chromatography. It discusses the high performance liquid chromatography (HPLC) and compares it with the gas chromatography (GC) method translation. It talks about the need to set performance goals to determine if an isocratic method...

  • Top 10 HPLC Column Myths. Majors, Ronald E. // LC-GC Europe;Jan2007, Vol. 20 Issue 1, p13 

    In any field there are often ‘misconceptions’ or ‘myths’ that are perpetuated and passed on to the next generation. These myths are often driven by a lack of understanding by practitioners of the real issues. In this instalment of ‘Column Watch’, the 10...

  • Column Pressure Considerations in Analytical HPLC. Majors, Ronald E. // LC-GC Europe;May2008, Vol. 21 Issue 5, p268 

    In this instalment of "Column Watch", columnist Ron Majors examines the role of pressure in high performance liquid chromatography (HPLC) from two viewpoints: the impact of the ultrahigh pressures encountered in ultrahigh-pressure liquid chromatography (UHPLC) on chromatographic parameters and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics