Comparison of potassium ion preference of potassium-sensing oligonucleotides, PSO-1 and PSO-2, carrying the human and Oxytricha telomeric sequence, respectively

Takenaka, Shigeori; Ueyama, Hiroyuki; Nojima, Takahiko; Takagi, Makoto
April 2003
Analytical & Bioanalytical Chemistry;Apr2003, Vol. 375 Issue 8, p1006
Academic Journal
Human [G3(TTAG3)3] and Oxytricha [G4(T4G4)3] telomere model oligonucleotides, PSO-1 and PSO-2, bearing two fluorophores, 6-carboxyfluorescein (6-FAM) and 6-carboxytetramethylrhodamine (6-TAMRA) at their 5′- and 3′-termini, respectively, were synthesized. Both of them can form an intramolecular antiparallel tetraplex upon addition of K+, and an enhanced fluorescence resonance energy transfer (FRET) was observed. PSO-1 showed a 43,000 times higher selectivity for K+ against Na+. Fluorometric and circular dichroism spectrophotometric studies revealed that this system is useful for the evaluation of the interaction of different telomeric repeat oligonucleotide sequences with metal ions.


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