Determination of phosphorus in small amounts of protein samples by ICP—MS

Becker, J. Sabine; Boulyga, Sergei F.; Pickhardt, Carola; Becker, J.; Buddrus, Stefan; Przybylski, Michael
February 2003
Analytical & Bioanalytical Chemistry;Feb2003, Vol. 375 Issue 4, p561
Academic Journal
Inductively coupled plasma mass spectrometry (ICP–MS) is used for phosphorus determination in protein samples. A small amount of solid protein sample (down to 1 µg) or digest (1–10 µL) protein solution was denatured in nitric acid and hydrogen peroxide by closed-microvessel microwave digestion. Phosphorus determination was performed with an optimized analytical method using a double-focusing sector field inductively coupled plasma mass spectrometer (ICP–SFMS) and quadrupole-based ICP–MS (ICP–QMS). For quality control of phosphorus determination a certified reference material (CRM), single cell proteins (BCR 273) with a high phosphorus content of 26.8±0.4 mg g–1, was analyzed. For studies on phosphorus determination in proteins while reducing the sample amount as low as possible the homogeneity of CRM BCR 273 was investigated. Relative standard deviation and measurement accuracy in ICP–QMS was within 2%, 3.5%, 11% and 12% when using CRM BCR 273 sample weights of 40 mg, 5 mg, 1 mg and 0.3 mg, respectively. The lowest possible sample weight for an accurate phosphorus analysis in protein samples by ICP–MS is discussed. The analytical method developed was applied for the analysis of homogeneous protein samples in very low amounts [1–100 µg of solid protein sample, e.g. β-casein or down to 1 µL of protein or digest in solution (e.g., tau protein)]. A further reduction of the diluted protein solution volume was achieved by the application of flow injection in ICP–SFMS, which is discussed with reference to real protein digests after protein separation using 2D gel electrophoresis. The detection limits for phosphorus in biological samples were determined by ICP–SFMS down to the ng g–1 level. The present work discusses the figure of merit for the determination of phosphorus in a small amount of protein sample with ICP–SFMS in comparison to ICP–QMS.


Related Articles

  • ICP-MS systems.  // Spectroscopy;Apr2006, Vol. 21 Issue 4, p54 

    The article evaluates two models of inductively-coupled plasma-mass spectroscopy instruments from Varian and offers information on design, key features and applications.

  • Hyphenated Techniques as Modern Detection Systems in Ion Chromatography. Wille, Andrea; Czyborra, Stefanie; Steinbach, Alfred // LC-GC Europe;Jul/Aug2009, Vol. 22 Issue 7, p8 

    This article describes the use of combined ion chromatography-mass spectrometry(IC-MS) and ion chromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) to analyse potentially harmful compounds.

  • Glass Expansion.  // Spectroscopy;Dec2009, Vol. 24 Issue 12, p29 

    The article presents company profile of Glass Expansion Inc. The company notes to manufacture sample introduction components for inductively coupled plasma (ICP) emission and mass spectrometers. It also develops unique manufacturing methods that results in high mechanical strength and...

  • A Pragmatic Approach to Managing Interferences in ICP-MS. Wilbur, Steve // Spectroscopy;May2008, Vol. 23 Issue 5, p18 

    The article discusses the concept of inductively coupled plasma-mass spectrometry (ICP-MS). This is capable of part-per-quadrillion detection limits under ideal conditions, most applications do not require this level of sensitivity and do not justify the cost associated with achieving it....

  • Milestone Inc.  // Spectroscopy;Dec2015, Vol. 30 Issue 12, p57 

    The article features Milestone Inc., a Shelton, Connecticut-based maker of spectroscopic instruments. Topics covered include brief company profile, major products/services, markets served by the company, description and location of its facilities and spectroscopic techniques supported by its...

  • Intracellular protein binding patterns of the anticancer ruthenium drugs KP1019 and KP1339. Heffeter, Petra; Böck, Katharina; Atil, Bihter; Reza Hoda, Mir; Körner, Wilfried; Bartel, Caroline; Jungwirth, Ute; Keppler, Bernhard; Micksche, Michael; Berger, Walter; Koellensperger, Gunda // Journal of Biological Inorganic Chemistry;Jun2010, Vol. 15 Issue 5, p737 

    The ruthenium compound KP1019 has demonstrated promising anticancer activity in a pilot clinical trial. This study aims to evaluate the intracellular uptake/binding patterns of KP1019 and its sodium salt KP1339, which is currently in a phase I–IIa study. Although KP1339 tended to be...

  • Inorganic Ventures.  // Spectroscopy;Dec2015, Vol. 30 Issue 12, p48 

    The article features Inorganic Ventures, a Christianburg, Virginia-based maker of spectroscopic instruments. Topics covered include brief company profile, major products/services, markets served by the company, description and location of its facilities and spectroscopic techniques supported by...

  • ICP rinse accessory.  // Spectroscopy;Feb2006, Vol. 21 Issue 2, p82 

    The article describes the Niagara Rapid Rinse inductively coupled plasma (ICP) spectrometry accessory from Pocasset, Massachusetts-based Glass Expansion Inc. Rapid Rinse is designed to shorten analysis cycle time of an unattended ICP spectrometer run. It begins the rinsing of the nebulizer and...

  • Determination of the titanium content of human transferrin by inductively-coupled plasma-atomic-emission spectroscopy. Cardona, Aracelis; Meléndez, Enrique // Analytical & Bioanalytical Chemistry;Dec2006, Vol. 386 Issue 6, p1689 

    We report a simple method that combines dialysis, as a purification method, with the multielement capability of ICP to determine the titanium-to-transferrin mole ratio at physiological pH, under buffer conditions. The method, by means of which titanium and transferrin are determined...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics