Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay

Goldman, Ellen R.; Anderson, George P.; Lebedev, Nikolai; Lingerfelt, Brian M.; Winter, Phan T.; Patterson, Charles H.; Mauro, J. Matthew
February 2003
Analytical & Bioanalytical Chemistry;Feb2003, Vol. 375 Issue 4, p471
Academic Journal
We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 μg L–1 (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 μg L–1 (0.05 ppb) for TNT dissolved in artificial seawater.


Related Articles

  • Competitive amperometric immunosensor based on covalent linking of a protein conjugate to dendrimer-functionalised nanogold substrate for the determination of 2,4,6-trinitrotoluene. Giannetto, Marco; Maiolini, Elisabetta; Ferri, Elida; Girotti, Stefano; Mori, Giovanni; Careri, Maria // Analytical & Bioanalytical Chemistry;Jan2013, Vol. 405 Issue 2/3, p737 

    A new amperometric immunosensor for 2,4,6-trinitrotoluene based on the working principle of competitive enzyme-linked immunosorbent assay was developed and characterised. An electrodeposited nanogold substrate was functionalised by deposition of self-assembled monolayers of 2-aminoethanethiol as...

  • Fluorescent polymer-porous silicon microcavity devices for explosive detection. Levitsky, I. A.; Euler, W. B.; Tokranova, N.; Rose, A. // Applied Physics Letters;1/22/2007, Vol. 90 Issue 4, p041904 

    Conjugated polymers entrapped in porous silicon microcavity have been studied as optical sensors for low volatility explosives such as trinitrotoluene. The fluorescence spectra of entrapped polymers were modulated by the microcavity via a spectral “hole” that matches the resonance...

  • Vertical variability of sound fields produced by underwater explosions at difference distances from the source. Galkin, O.; Popov, R.; Tuzhilkin, Yu. // Acoustical Physics;Nov2008, Vol. 54 Issue 6, p823 

    To determine the spatial variability of sound fields in the Black Sea, correlation of multipath signals received by vertically separated hydrophones is investigated. The signals are produced by underwater explosions of trinitrotoluene charges. Dependence of the sound field variability on the...

  • Explosives: A microsensor for trinitrotoluene vapour. Pinnaduwage, L.A.; Gehl, A.; Hedden, D.L.; Muralidharan, G.; Thundat, T.; Lareau, R.T.; Sulchek, T.; Manning, L.; Rogers, B.; Jones, M.; Adams, J.D. // Nature;10/2/2003, Vol. 425 Issue 6957, p474 

    Sensing devices designed to detect explosive vapours are bulky, expensive and in need of technological improvement - dogs remain the most effective detectors in the fight against terrorism and in the removal of land-mines. Here we demonstrate the deflagration of trinitrotoluene (TNT) in a small...

  • REMUS100 AUV with an Integrated Microfluidic System for Explosives Detection. Adams, A. A.; Charles, P. T.; Deschamps, J. R.; Kusterbeck, A. W. // NRL Review;2011, p95 

    The article investigates the presence of nitroaromatic materials in the U.S. marine environment using high-throughput microfluidic immunosensors (HTMIs) to perform displacement-based immunoassays onboard Hydroid REMUS100 autonomous underwater vehicle (AUV). The researchers immobilized antibodies...

  • Attenuation of TNT in seawater microcosms. Harrison, I.; Vane, C. H. // Water Science & Technology;2010, Vol. 61 Issue 10, p2531 

    The ability of two differing marine sediments (one clayey, the other sandy) to attenuate the explosive 2,4,6-trinitrotoluene (TNT), dissolved in intertidal seawater from the eastern English coast of the North Sea, was examined using aerobic microcosms. Analysis of the seawater from the...

  • Rapid Fluorescence Polarization Immunoassay for Highly Sensitive Detection of 2,4,6-Trinitrotoluene. WENZONG LU; YUAN NI; GUANGFENG JIA; HAIXIAN PAN; CHEN ZHAO // Asian Journal of Chemistry;2014, Vol. 26 Issue 3, p773 

    Detection of explosives is of great significance in public security programmer and environmental science. In the present work, a fluorescence polarization immunoassay based on a monoclonal antibody for the detection of 2,4,6-trinitrotoluene was established and optimized. Fluorescein-labeled...

  • TNT.  // American Heritage Student Science Dictionary;2009, p346 

    A definition for the term "TNT" is presented. It is the abbreviated term for the chemical trinitrotoluene. It is a yellow, crystalline compound used mainly as an explosive.

  • Death Underground.  // Time;1/19/1953, Vol. 61 Issue 3, p27 

    The article reports on the death of 44 Reds and the destruction of the tunnel under the north slope of Anchor Hill, located east coast of Korea and in the northernmost point of the Eight Army's 155-mile front, following an explosion. The purpose of the South Koreans for digging in on top of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics