TITLE

Unilamellar liposomes covalently coupled on silica gel for liquid chromatography

AUTHOR(S)
Xiqin Mao; Liang Kong; Xin Li; Baochuan Guo; Hanfa Zou
PUB. DATE
February 2003
SOURCE
Analytical & Bioanalytical Chemistry;Feb2003, Vol. 375 Issue 4, p550
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Silica gel was used as a support for the covalent coupling of liposomes, which could overcome drawbacks of soft gel beads in column efficiency and separation speed. The influences of the concentration of added dimethylaminopyridine and reaction time on the chloroformate activation reaction of silica gel were investigated. Temperature and pH for covalent coupling of liposomes on the activated silica gel were also optimized. Experimental results indicated that the stability of the covalently coupled liposome columns was obviously superior to that of the noncovalently coated liposome columns but the selectivity of both columns was basically identical. Separation and analysis of a crude extract of a traditional Chinese medicine Ligusticum Wallichii and a mixture of small peptides on both columns further support this conclusion.
ACCESSION #
15125545

 

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