Determination of ginkgolide A, B, and bilobalide in biloba L. extracts by microdialysis-HPLC

Houn-Lin Chiu; Hui-Ya Lin; Thomas Ching-Cherng Yang
June 2004
Analytical & Bioanalytical Chemistry;Jun2004, Vol. 379 Issue 3, p445
Academic Journal
A method has been developed for measuring ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts by using a self-assembled microdialysis device coupled on-line to high-performance liquid chromatography. The optimal conditions for dialysis efficiency and chromatographic analysis were investigated. The results show that the dialysis efficiencies for ginkgolide A, B, and bilobalide are 97.8–100.4%. For the spike concentration of 45 mg L-1, the recoveries of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts were at 97–100.7% with 3.1–4.7% RSD. The proposed method has therefore been shown to be applicable to the analysis of ginkgolide A, B, and bilobalide in ginkgo biloba L. extracts.


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