Separation of fatty acid methyl esters by comprehensive two-dimensional supercritical fluid chromatography with packed columns and programming of sampling duration

Hirata, Yukio; Sogabe, Ito
April 2004
Analytical & Bioanalytical Chemistry;Apr2004, Vol. 378 Issue 8, p1999
Academic Journal
Fatty acid methyl esters from various fats and oils were separated by comprehensive two-dimensional supercritical fluid chromatography with conventional packed columns and FID detection. The first dimension was a silica gel column and the second dimension was an ODS column. This combination was largely orthogonal for the separation of fatty acid methyl esters. The first dimension separations were primarily based on the number of double bonds while the second dimension separations were based on the chain length. The highly-ordered chromatograms and improved resolution allowed the easy detection and identification of minor components. Although the first dimension separations were performed under isobaric conditions where the peak width increased in proportion to the retention, the programming of the sampling duration allowed us to maintain the optimum re-injection frequency (3–4 times) per peak into the second dimension and so to minimize the total analysis time without deteriorating the resolution.


Related Articles

  • Fatty acid methyl and ethyl esters as well as wax esters for evaluating the quality of olive oils. Biedermann, Maurus; Bongartz, Annette; Mariani, Carlo; Grob, Koni // European Food Research & Technology;Nov2008, Vol. 228 Issue 1, p65 

    A promising correlation between chemical analysis and sensorial evaluation was confirmed: extra virgin olive oils with low contents of methyl and ethyl esters of fatty acids as well as straight chain wax esters were sensorially evaluated as being of high quality, whereas some with high contents...

  • MTBE Sees Strongest Growth Outside the US. Richards, Don // Chemical Market Reporter;10/29/2001, Vol. 260 Issue 16, p4 

    Discusses the prospects for methyl tertiary-butyl ether (MTBE) demand outside the United States (U.S.) as of October 2001. Statistics on the amount of MTBE used in California everyday in reformulated gasoline; Outlook on the possible scenarios related to the planned phase out of MTBE in...

  • MTBE's Effects. Medlin, Jennifer // Environmental Health Perspectives;Aug2000, Vol. 108 Issue 8, pA 371 

    Reports on a study by Nancy Fiedler and colleagues at the University of Medicine and Dentistry of New Jersey--Robert Wood Johnson Medical School in Piscataway, New Jersey of the health effects of controlled exposures to MTBE (methyl tertiary butyl ether). Comparison of the symptoms,...

  • Methyl 2,4-dihydroxy-3,6-dimethylbenzoate. Zviely, Michael // Perfumer & Flavorist;Jan2011, Vol. 36 Issue 1, p54 

    The article discusses methyl 2,4-dihydroxy- 3,6-dimethylbenzoate, a white to off-white crystalline powder that is essential as an fixative, powdery and woody-sweet. Methyl 2,4-dihydroxy- 3,6-dimethylbenzoate also has an odor character of treemoss and oak. The capability of the molecular base of...

  • Metro New York opt-out looms. Morris, Gregory DL // Chemical Week;1/28/1998, Vol. 160 Issue 4, p48 

    Focuses on the permission on the counties in New Jersey and Connecticut which are part of the New York Metropolitan Statistical Area to withdraw on their participation in the federal winter oxygenated fuels program. Expected impact on the market for methyl tert-butyl ether (MTBE), primary...

  • product focus.  // Chemical Week;10/8/2003, Vol. 165 Issue 36, p28 

    Provides information on the market status of methyl tert-butyl ether (MTBE) as of October 8, 2003. Prices of MTBE in U.S., Europe and Asia; Outlook for the product; Way of producing MTBE.

  • MTBE Prices Fall.  // Chemical Week;10/31/2001, Vol. 163 Issue 40, p37 

    Reports the decline in the spot prices of methyl tert-butyl ether in Europe as of October 31, 2001.

  • Controlled Human Exposure to Methyl Tertiary Butyl Ether in Gasoline: Symptoms, Psychophysiologic and Neurobehavioral Responses of Self-Reported Sensitive Persons. Fiedler,, Nancy; Kelly-McNeil,, Kathie; Mohr,, Sandra; Lehrer,, Paul; Opiekun,, Richard E.; Lee, Chia-Wei; Wainman,, Tom; Hamer,, Robert; Weisel,, Clifford; Edelberg,, Robert; Lioy, Paul J. // Environmental Health Perspectives;Aug2000, Vol. 108 Issue 8, p753 

    Reports on a study to determine the effects of exposure to methyl tertiary butyl ether (MTBE) in gasoline. Addition of the ether to gasoline in order to control carbon monoxide standards; Comparison of reactions in people with self-reported sensitivity to the ether and those without...

  • An efficient multigram synthesis of juglone methyl ether. Cui, Jia-hua; Cui, Qing; Zhang, Qi-jing; Li, Shao-shun // Journal of Chemical Research;Sep2015, Vol. 39 Issue 9, p553 

    Based on the regioselective oxidation of 1,4,5-trimethoxynaphthalene by cerium (IV) ammonium nitrate, an efficient synthesis of juglone methyl ether has been achieved with high overall yield (74%) and good purity (98.6%). Compared with the reported methods, the reaction conditions are milder and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics