In-capillary micro solid-phase extraction and capillary electrophoresis separation of heterocyclic aromatic amines with nanospray mass spectrometric detection

Viberg, Peter; Nilsson, Staffan; Skog, Kerstin
April 2004
Analytical & Bioanalytical Chemistry;Apr2004, Vol. 378 Issue 7, p1729
Academic Journal
A miniaturised technique to analyse and detect heterocyclic aromatic amines (HAs) using micro solid-phase extraction (μSPE) coupled on-line (in-capillary) to capillary electrophoresis (CE) separation with nanospray (nESI) mass spectrometry (MS) detection has been developed. HAs are mutagenic and carcinogenic compounds formed at low levels in protein-rich food during cooking. Due to the low concentrations of HAs and the high complexity of the matrix in which they exist, sensitive and selective analytical methods are required for quantification. μSPE was performed on a packed bed of C18 particles inside the CE capillary, which minimised the dead volume. The on-line coupling of SPE, CE and nESI-MS reduced the time for extraction and identification to less than half an hour, which will allow for screening of several samples per day. The new technique provides short analysis time, low sample and solvent consumption, and HAs in standard solutions were easily detected at 12–17 fmol injections, and in spiked urine samples at 750–810 fmol injections.


Related Articles

  • Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes. Weissinger, Eva M.; Wittke, Stefan; Kaiser, Thorsten; Haller, Hermann; Bartel, Sebastian; Krebs, Ronald; Golovko, Igor; Rupprecht, Harald D.; Haubitz, Marion; Hecker, Hartmut; Mischak, Harald; Fliser, Danilo // Kidney International;Jun2004, Vol. 65 Issue 6, p2426 

    Proteomic patterns established with capillary electrophoresis and mass spectrometry for diagnostic purposes. Background. Proteomics applied in large scale may provide a useful diagnostic tool. Methods. We developed an online combination of capillary electrophoresis with mass spectrometry,...

  • Mass appeal: metabolite identification in mass spectrometry-focused untargeted metabolomics. Dunn, Warwick; Erban, Alexander; Weber, Ralf; Creek, Darren; Brown, Marie; Breitling, Rainer; Hankemeier, Thomas; Goodacre, Royston; Neumann, Steffen; Kopka, Joachim; Viant, Mark // Metabolomics;Mar2013 Supplement, Vol. 9, p44 

    Metabolomics has advanced significantly in the past 10 years with important developments related to hardware, software and methodologies and an increasing complexity of applications. In discovery-based investigations, applying untargeted analytical methods, thousands of metabolites can be...

  • Metabolic and morphological changes of an oil accumulating trebouxiophycean alga in nitrogen-deficient conditions. Ito, Takuro; Tanaka, Miho; Shinkawa, Haruka; Nakada, Takashi; Ano, Yoshitaka; Kurano, Norihide; Soga, Tomoyoshi; Tomita, Masaru // Metabolomics;Mar2013 Supplement, Vol. 9, p178 

    Oil-rich algae have promising potential for a next-generation biofuel feedstock. Pseudochoricystis ellipsoidea MBIC 11204, a novel unicellular green algal strain, accumulates a large amount of oil (lipids) in nitrogen-deficient (-N) conditions. Although the oil bodies are easily visualized by...

  • Visualization of Spatiotemporal Energy Dynamics of Hippocampal Neurons by Mass Spectrometry during a Kainate-Induced Seizure. Sugiura, Yuki; Taguchi, Ryo; Setou, Mitsutoshi // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    We report the use of matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry combined with capillary electrophoresis (CE) mass spectrometry to visualize energy metabolism in the mouse hippocampus by imaging energy-related metabolites. We show the distribution patterns of...

  • Evaluation of different PCR-based approaches for the identification and typing of environmental enterococci. Domenico Pangallo; Hana Drahovska; Jana Harichova; Edita Karelova; Katarina Chovanova; Jana Aradska; Peter Ferianc; Jozef Timko // Antonie van Leeuwenhoek;Feb2008, Vol. 93 Issue 1/2, p193 

    Abstract  The aim of the work was the evaluation of different PCR-based methods to found an appropriate identification and typing strategy for environmental enterococci. Environmental enterococci were isolated mainly from surface- and waste-waters. Species identification was...

  • On-column sample enrichment for the high-sensitivity sheath-flow CE-MS analysis of peptides. Sandra, K.; Lynen, F.; Devreese, B.; Van Beeumen, J.; Sandra, P. // Analytical & Bioanalytical Chemistry;Jul2006, Vol. 385 Issue 4, p671 

    A sample enrichment technique to increase sensitivity in capillary electrophoresis–mass spectrometry (CE-MS) is described. Peptides or glycopeptides are retained and concentrated on a short (3–5-mm) reversed-phase (C18) packed-bed situated in the fused-silica separation capillary...

  • Optimization of a rapid capillary electrophoresis ESI-IT tandem mass spectrometry method for the analysis of short-chain carnitines in human plasma. Desiderio, Claudia; De Rossi, Antonella; Inzitari, Rosanna; Mancinelli, Angelo; Rossetti, Diana Valeria; Castagnola, Massimo; Messana, Irene // Analytical & Bioanalytical Chemistry;Mar2008, Vol. 390 Issue 6, p1637 

    A capillary electrophoresis (CE) method coupled to electrospray ionization ion trap tandem mass spectrometry (ESI-IT-MS/MS) is described for the rapid analysis of carnitine, acetylcarnitine, and propionylcarnitine in human plasma. Optimization of the procedure was achieved by a reduced sample...

  • Optimization of CDTE Quantum Dots Synthesis Using Capillary Zone Electrophoresis. Clarot, Igor; Wolpert, Cçcile; Morosini, Vincent; Schneider, Raphaël; Balan, Lavinia; Diez, Laurent; Leroy, Pierre // Current Nanoscience;May2009, Vol. 5 Issue 2, p154 

    Water-soluble, thiol protected cadmium telluride CdTe quantum dots (QDs) exhibit size dependent excitation/emission wavelength tunability. The heating period ensuring the growth of CdTe nanocrystals capped with thioglycolic acid (CdTe@TGA) in aqueous solution is critical to reach a...

  • Characterization of Latex Allergenic Components by Capillary Zone Electrophoresis and N-Terminal Sequence Analysis. Hung-Dar Chen, Robert K.; Chao-Ling Chen; Shih-Wen Huang; Hsiang-Fu Kung; Hao-Chia Chen // Journal of Biomedical Science;1998, Vol. 5 Issue 6, p421 

    In a previous study, protein components purified from latex gloves that elicited allergenic reactions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and yielded apparent molecular weights of 14, 22, 30, 34, 46, and 58 kD. These allergenic components...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics