Kinetic method for acetylsalicylic acid determination based on its inhibitory effect upon the catalytic decomposition of H2O2

Mureşanu, Claudia; Copolovici, Lucian
April 2004
Analytical & Bioanalytical Chemistry;Apr2004, Vol. 378 Issue 7, p1868
Academic Journal
The catalytic reaction of catalytic was investigated, by means of a Clark oxygen sensor, in the presence of various concentrations of acetylsalicylic acid. Michaelis-Menten kinetic parameters were determined from Lineweaver- Burk plots, obtained in the absence and in the presence of the inhibitor. The inhibition pattern, suggested by the Lineweave- Burk plots, corresponds to a fully mixed inhibition mechanism. A kinetic method, based on the indicator reaction: H2O2 [this equation can't be represented in ASCII.txt]H2O+0.502, was developed for the quantitative determination of acetylsalicylic acid. Calibration graphs of the reciprocal value of first-order rate constant versus acetylsalicylic concentration covered the concentration range (2.99-19.98)×10-4 mol/L, while the detection limit was 4.12×10-4 mol/L acetylsalicylic acid with a standard deviation of 2.1×10-5 mol/L.


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