Functionalised electrode array for the detection of nitric oxide released by endothelial cells using different NO-sensing chemistries

Oni, Joshua; Pailleret, Alain; Isik, Sonnur; Diab, Nizam; Radtke, Ina; Blöchl, Andrea; Jackson, Michael; Bedioui, Fethi; Schuhmann, Wolfgang
March 2004
Analytical & Bioanalytical Chemistry;Mar2004, Vol. 378 Issue 6, p1594
Academic Journal
In a preliminary study aimed at developing strategies for the simultaneous detection of various biologically important molecules, a procedure is described that allows the electrochemical detection of nitric oxide (NO) released by a population of human umbilical vein endothelial cells (HUVEC) by using an array of electrodes comprising three individually addressable electrodes. Each electrode in the array was modified with a different NO-sensitive electrocatalyst, thereby demonstrating the possibility of modifying the individual electrodes in an array with different sensing chemistries. This study opens a doorway to the development of arrays of electrodes for the simultaneous detection of multiple analytes in a complex environment by suitably tailoring the sensitivity and selectivity of each electrode in the array to a specific analyte in the test medium.


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