TITLE

A one-step immunochromatographic assay for detecting ginsenosides Rb1 and Rg1

AUTHOR(S)
Putalun, Waraporn; Fukuda, Noriko; Tanaka, Hiroyuki; Shoyama, Yukihiro
PUB. DATE
March 2004
SOURCE
Analytical & Bioanalytical Chemistry;Mar2004, Vol. 378 Issue 5, p1338
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An immunochromatographic strip test has been developed for detecting ginsenosides Rb1 (G-Rb1) and Rg1 (G-Rg1). This qualitative assay system is useful as a rapid screening method for detecting G-Rb1 and G-Rg1 in plants and plant preparations. Our assay is a competitive immunoassay that uses anti-G-Rb1 and anti-G-Rg1 monoclonal antibodies (MAbs) and a detection reagent that contains colloidal gold particles coated with anti-G-Rb1 and anti-G-Rg1 MAbs. Detection limits are 2 μg mL-1 for both G-Rb1 and G-Rg1.
ACCESSION #
15124901

 

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