Production of certified reference materials for mycotoxins: IRMM’s view on the assessment of uncertainties

Josephs, Ralf Dieter; Koeber, Robert; Linsinger, Thomas Peter Josef; Bernreuther, Alexander; Ulberth, Franz; Schimmel, Heinz
March 2004
Analytical & Bioanalytical Chemistry;Mar2004, Vol. 378 Issue 5, p1190
Academic Journal
Analytical difficulty and the economic importance of controlling mycotoxin levels in food and feed led the Community Bureau of Reference (BCR) to prepare a series of certified reference materials (CRM) for various mycotoxins. Because of the wide acceptance of these CRM and the need to ensure the comparability and traceability of measurements in the future it is necessary to prepare and certify new batches of mycotoxin reference materials (RM). In the following text two different approaches for evaluation of the characterisation uncertainty of CRM will be compared using the certification of aflatoxin M1 (AfM1) in milk powder as an example. The conventional approach is based on evaluation of characterisation exercise data; the alternative approach is based on measurement uncertainties of the employed analytical methods. Because laboratories are using totally different approaches to estimate the measurement uncertainties, combination of the uncertainties obtained from the participating laboratories was not recommended. Therefore, a new integrated approach for assessment of the measurement uncertainties of the analytical methods on the basis of additional data collected during the characterisation exercise will be described. The conventional approach was found to be the most appropriate and economical approach to evaluate the characterisation uncertainty as a characterisation exercise must be performed anyway to establish the property values of candidate (C)RM, irrespective of whether or not reliable measurement uncertainties can be provided by the laboratories. An integrated approach for assessment of measurement uncertainties based on additional characterisation data as applied here to enable use of an uncertainty-based approach provides more information but is too time-consuming and cost-intensive to become common practice.


Related Articles

  • Analysis for Mycotoxins: The Chemist's Perspective. Jarvis, Bruce B. // Archives of Environmental Health;Aug2003, Vol. 58 Issue 8, p479 

    Examines the chemical methods available for mycotoxin analysis. Isolation and characterization of mycotoxins; Procedures for the analysis of mycotoxins; Limitations of chemical analyses.

  • QuickTox Kit for QuickScan Aflatoxin. BAILEY, CHERYL; DAVIS, ALAN H. // Journal of AOAC International;Sep/Oct2012, Vol. 95 Issue 5, p1460 

    The article presents a study which examined the performance of the QuickTox Kit for QuickScan Aflatoxin, which uses lateral flow technology and a reader-based system for quantitative determination of total aflatoxins. Corn sample naturally contaminated with aflatoxins in internal and independent...

  • Comparison of mycotoxin profiles among cereal samples from eastern Canada. Campbell, Harold; Choo, Thin Meiw; Vigier, Bernard; Underhill, Lynne // Canadian Journal of Botany;May2002, Vol. 80 Issue 5, p526 

    The relative susceptibilities of major cereal species to mycotoxin contamination have rarely been studied in eastern Canada or elsewhere. The concentration of 13 mycotoxins in 673 corn (Zea mays L.), 99 wheat (Triticum aestivum L.), 116 barley (Hordeum vulgare L.), and 73 oat (Avena sativa L.)...

  • MYCOTOXICOLOGICAL TESTS FROM THE ASPECT OF THE HACCP SYSTEM AND LEGISLATION. JakŢic, Sandra M.; Stojanov, Igor M.; Baloš, Milica M. Živkov; Mašić, Zoran S.; Mihaljev, Željko A.; Abramović, Biljana F. // Matica Srpska Proceedings for Natural Sciences / Zbornik Matice ;2011, Vol. 121, p95 

    Mycotoxins, as secondary metabolic products of molds, are common contaminants of raw feed materials and compound feeds. Depending on the agro-meteorological and storage conditions, molds can contaminate grains and produce mycotoxins in the field, before and after harvest and during storage. Way...

  • DIAGNOSIS OF MYCOTOXICOSES IN VETERINARY MEDICINE. Nešić, Ksenija D.; Dimić, Dobrila P. Jakić -; Jojić-Malićević, Ljiljana M.; Savić, BoŽidar M. // Matica Srpska Proceedings for Natural Sciences / Zbornik Matice ;2011, Vol. 121, p221 

    The problem of mycotoxin presence in animal feed and the consequences that arise due to this, represent a great challenge for anyone encountering them. In the chain which includes studies from prevention to treatment, a very important place and a frequent source of confusion is the process of...

  • A Three-Year Survey on the Worldwide Occurrence of Mycotoxins in Feedstuffs and Feed. Rodrigues, Inês; Naehrer, Karin // Toxins;Sep2012, Vol. 4 Issue 9, p663 

    Between January 2009 and December 2011, a total of 7049 corn, soybean/soybean meal, wheat, dried distillers grains with solubles (DDGS) and finished feed samples were analyzed for the occurrence of aflatoxins (Afla), zearalenone (ZEN), deoxynivalenol (DON), fumonisins (FUM) and ochratoxin A...

  • Development of a Multiresidue Method for Mycotoxin Analysis in Feeds and Grains.  // LC-GC North America;Jun2004 Supplement, Vol. 22, p41 

    Presents the development of a multiresidue method for mycotoxin analysis in feeds and grains. Definition and characteristics of mycotoxins; Analysis of a method that allows five families of toxins in a single chromatographic run; Illustration of multiresidue method.

  • Qualitative changes of rye grain and flour after Fusarium spp. contamination evaluated by standard methods and system Mixolab. PAPOUŠKOVÁ, Ludmila; CAPOUCHOVÁ, Ivana; DVOŘÁČEK, Václav; KONVALINA, Petr; JANOVSKÁ, Dagmar; VEPŘÍKOVÁ, Zdena; ŠKEŘÍKOVÁ, Alena; ZRCKOVÁ, Monika // Zemdirbyste-Agriculture;2015, Vol. 102 Issue 4, p397 

    The effect of Fusarium spp. infestation on rheological quality of rye flour in different growing systems (organic and conventional) was measured by the rheological system Mixolab. The content of mycotoxins (DON, D3G, 3-ADON and ZON) and standard technological quality parameters of grain were...

  • Analysis of Fusarium Toxins Using LC-MS-MS: Application to Various Food and Feed Matrices. Biselli, Scarlett; Hartig, Lutz; Wegner, Heiner; Hummert, Christian // LC-GC North America;Apr2005, Vol. 23 Issue 4, p404 

    Co-occurrence of several mycotoxins (deoxynivalenol, zearalenone, T-2- toxin, HT-2 toxin) produced by field fungi, such as Fusarium graminearum and FusarIum culmorum, requires several analysis methods for their characterization. A reliable method for the determination of type A- and B-...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics