TITLE

The need for reference materials when monitoring nitrate intake

AUTHOR(S)
Castanheira, Isabel; Oliveira, Luisa; Valente, Ana; Alvito, Paula; Costa, Helena S.; Alink, Anton
PUB. DATE
March 2004
SOURCE
Analytical & Bioanalytical Chemistry;Mar2004, Vol. 378 Issue 5, p1232
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Whether dietary exposure to nitrate metabolites is detrimental or beneficial to human health has long been a matter of controversy. In spite of no consistent epidemiological evidence, nitrate metabolites are associated with the formation of carcinogenic-nitrosamines and gastric cancer. Furthermore, recent studies demonstrate that ingested nitrate plays a role in host defence against gastrointestinal pathogenic bacteria. Analytical values of nitrate content in foods are essential for estimating nitrate intake. The analytical process is of paramount importance for assessing human nitrate exposure and for establishing a link between these exposures and the current and future observed health effects. Therefore, the quality assurance of the measurement process is crucial to obtaining reliability, comparability and traceability of results. Certified Reference Materials (CRM) should play a role in the consistency of the measurement process. However, the availability of nitrate CRMs is still poor. When food monitoring is demanded, an approach could be to use In House Reference Materials (IHRM), prepared at a high metrological level, and all preparation steps should be quality driven. IHRMs were prepared, and available CRMs were used to provide traceability of the process. The homogeneity of IHRM was evaluated using an appropriate statistical design. The stability was monitored using an isochronous method. The material shelf life and storage conditions are presented. HPLC was optimised for the determination of nitrates in four vegetable categories. When a suite of IHRMs were used, the response of the HPLC system was linear over the range 1 to 8 mg L–1. The detection limit for these compounds was 0.2 μg L-1 and the determination limit 1.2 μg L-1. The relationship between measurement uncertainty and critical points of the analytical process is presented. The differences in observed relative uncertainty between food categories could reflect current limitations in the food matrix reference materials.
ACCESSION #
15124886

 

Related Articles

  • CME Questions.  // Infectious Disease Alert;Feb2009, Vol. 28 Issue 5, p22 

    A quiz about pathogenic bacteria and bacterial diseases discussed within the issue.

  • Improving Nasopharyngeal Cultures in Office Practice. Menachof, Louis W.; Mount, Robert // Clinical Pediatrics;Oct1975, Vol. 14 Issue 10, p913 

    The article discusses about improving nasopharyngeal cultures in practice of pediatric. In office pediatric practice, throat and urine cultures are the most common bacteriologic procedures performed. Most of the recognized pathogenic bacteria are either aerobic, facultatively anaerobic, or...

  • HpaC Controls Substrate Specificity of the Xanthomonas Type III Secretion System. Lorenz, Christian; Schulz, Steve; Wolsch, Thomas; Rossier, Ombeline; Bonas, Ulla; Büttner, Daniela // PLoS Pathogens;Jun2008, Vol. 4 Issue 6, p1 

    The Gram-negative bacterial plant pathogen Xanthomonas campestris pv. vesicatoria employs a type III secretion (T3S) system to inject bacterial effector proteins into the host cell cytoplasm. One essential pathogenicity factor is HrpB2, which is secreted by the T3S system. We show that secretion...

  • Cytolethal Distending Toxin: A Bacterial Bullet Targeted to Nucleus. Ohara, Masaru; Oswald, Eric; Sugai, Motoyuki // Journal of Biochemistry;Oct2004, Vol. 136 Issue 4, p409 

    Cytolethal distending toxin (Cdt) is a newly added member of bacterial protein toxins that hijack the control system of eukaryotic cells. Cdts are produced by several pathogenic bacteria causing chronic infectious diseases. They are composed of three subunits, CdtA, CdtB and CdtC, which together...

  • Doxycycline Treatment of Lymphatic Filariasis.  // Clinical Infectious Diseases;9/15/2005, Vol. 41 Issue 6, preceding p1 

    The article cites a study on doxycycline treatment of Lymphatic Filariasis. It reports that Wolbachia species are endosymbiotic bacteria commonly found in arthropods but also present in the human filarial nematodes. Their closest phylogenetic relatives include Rickettsia, Ehrlichia, and...

  • Prokaryotic toxin–antitoxin stress response loci. Gerdes, Kenn; Christensen, Susanne K.; Løbner-Olesen, Anders // Nature Reviews Microbiology;May2005, Vol. 3 Issue 5, p371 

    Although toxin–antitoxin gene cassettes were first found in plasmids, recent database mining has shown that these loci are abundant in free-living prokaryotes, including many pathogenic bacteria. For example, Mycobacterium tuberculosis has 38 chromosomal toxin–antitoxin loci,...

  • ENVIRONMENTAL SAMPLING OF SELECTED PUBLIC HEALTH NURSING BAG CONTENTS. Geissinger, Amy D. // American Journal of Public Health;Sep1971, Vol. 61 Issue 9, p1853 

    A bacteriological study was made to see whether selected contents of public health nursing bags were contaminated by pathogens common in a "normal" environment. No pathogenic bacteria were identified, but three nonpathogenic organisms were found. More attention was given to protection from...

  • Genomes of the Most Dangerous Epidemic Bacteria Have a Virulence Repertoire Characterized by Fewer Genes but More Toxin-Antitoxin Modules. Georgiades, Kalliopi; Raoult, Didier // PLoS ONE;2011, Vol. 6 Issue 3, p1 

    Background: We conducted a comparative genomic study based on a neutral approach to identify genome specificities associated with the virulence capacity of pathogenic bacteria. We also determined whether virulence is dictated by rules, or if it is the result of individual evolutionary histories....

  • In silico identification of potential therapeutic targets in Clostridium botulinum by the approach subtractive genomics. Koteswara Reddy, G.; Nagamalleswara Rao, K.; Krishna, B. Phani Rama; Aravind, S. // International Journal of Bioinformatics Research;2010, Vol. 2 Issue 2, p12 

    The completion of genome sequences of pathogenic bacteria and the completion of human genome project has provided lot amount of data that can be utilized to design vaccines and drug targets. One of the recently adopting strategies for drug designing is based on comparative genomics approach, in...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics