Protease sensors for bioimaging

Funovics, Martin; Weissleder, Ralph; Ching-Hsuan Tung
November 2003
Analytical & Bioanalytical Chemistry;Nov2003, Vol. 377 Issue 6, p956
Academic Journal
Optical imaging of specific molecular targets and pathways in vivo has recently become possible through continued developments in imaging equipment, reconstruction algorithms, and more importantly the availability of imaging reporter molecules. These reporter molecules encompass photoproteins expressed in vivo and exogenously administered probes detectable by fluorescence and/or bioluminescence imaging. One particularly enticing aspect of optical imaging is the ability to design activatible probes with inherent amplification. This review summarizes our experience in developing novel near-infrared fluorescent (NIRF) imaging agents that report on protease activities. These agents are designed to be biocompatible, highly activatible, and able to produce bright NIRF following protease cleavage.


Related Articles

  • Lights! Camera! Action! Netterwald, James // Drug Discovery & Development;Feb2008, Vol. 11 Issue 2, p18 

    The article focuses on the usefulness of live-cell imaging. Experts claim that the main advantage of live-cell imaging is that it can yield information about the dynamics of cellular processes. The author states that a good lighting in the form of a good reporter molecule is needed to take great...

  • Restrahlen to T-Rays — 100 Years of Terahertz Radiation. Kimmitt, M.F. // Journal of Biological Physics;Apr2003, Vol. 29 Issue 2/3, p77 

    Although a reliable source of subpicosecond terahertz (THz) pulses, or T-rays, was demonstrated in the late 1980s, it was not until 1995, when they were used for imaging, that their full potential was realized. However, much of the publicity at that time seemed to suggest that very little...

  • High-speed cameras target motion analysis. Wilson, Andrew // Vision Systems Design;Dec2004, Vol. 9 Issue 12, p39 

    Focuses on the important developments in high-speed imaging systems which are useful for ensuring the quality of products produced by competitive firms. Benefits from the introduction of CMOS imaging sensors used in cameras; Functions of frame grabbers in advanced imaging systems; Advantages of...

  • Split Aperture Imaging for High Dynamic Range. Aggarwal, Manoj; Ahuja, Narendra // International Journal of Computer Vision;Jun2004, Vol. 58 Issue 1, p7 

    Most imaging sensors have limited dynamic range and hence are sensitive to only a part of the illumination range present in a natural scene. The dynamic range can be improved by acquiring multiple images of the same scene under different exposure settings and then combining them. In this paper,...

  • Lumidigm adds multispectral imaging to fingerprint sensor. Kincade, Kathy // Laser Focus World;Dec2004, Vol. 40 Issue 12, p53 

    This article reports that Lumidigm Inc. has been awarded a technology research grant to fund further development of a new biometric sensor based on the company's multispectral imaging technology. The company has created novel biometric and antispoofing technologies, based upon optical...

  • Canon Details its Immersion Roadmap. Chappell, Jeff // Electronic News;7/18/2005, Vol. 51 Issue 29, pN.PAG 

    Reports on the plan of Canon Inc. to produce a hyper numerical aperture immersion tool. Possible features of the tool; Efforts of the company to explore extreme ultraviolet options for immersion technology; Requirement for installing the tool.

  • Matching Lenses and Sensors. Hollows, Greg; Singer, Stuart // Vision Systems Design;Mar2009, Vol. 14 Issue 3, p13 

    The article discusses the factors to consider in image sensor/lens combination such as diffraction, lens aperture, focal length and the wavelength of light. It seeks to show manufacturers how lenses limit the performance of imaging systems through these factors. Manufacturers will thus be...

  • PRODUCTS.  // Rock Products;Jan/Feb2006 Cement Supplement, p23 

    The article offers several products for the cement industry. The list include the bag cleaning conveyor station from Chantland-MHS, FilterSense's Liquid Mist Flow Monitors, imaging and sensing technology from IST Imaging Systems Group, PortoVeyor bulk bag unloaders from Cyclonaire Corp. and...

  • MICRO-MINIATURE SPLIT STIRLING LINEAR CRYCOOLER. Veprik, A.; Zehtzer, S.; Vilenchik, H.; Pundak, N. // AIP Conference Proceedings;4/9/2010, Vol. 1218 Issue 1, p363 

    Novel tactics for rescue, surveillance, reconnaissance, force protection, perimeter security, navigation and targeting often involve the use of miniature infrared imagers, where the cooled imaging systems are known to be superior to their uncooled rivals in terms of working range, resolution and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics