Wolfish behavior...the successful scientist as leader of a pack or a lone wolf

Fetzer, J.
November 2003
Analytical & Bioanalytical Chemistry;Nov2003, Vol. 377 Issue 5, p785
Academic Journal
The article presents information on the determination of serum D-lactic and L-lactic acids in normal subjects and diabetic patients by column-switching HPLC with pre-column fluorescence derivatization. Lactic acid in mammals is mainly composed of the L-form which is produced from pyruvic acid under anaerobic conditions. But its optical isomer, D-lactic acid, also exists in mammals even though the amount is approximately 1.0% relative to L-lactic acid. For the determination of D-lactic acid in blood, serum, or plasma, the reported methods have mostly utilized D-lactic acid dehydrogenase, which transforms D-lactic acid to pyruvic acid.


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