TITLE

Analysis of meso-2,3-dimercaptosuccinic acid in human urine by capillary electrophoresis using direct injection

AUTHOR(S)
Xiaofeng Zhu; Lever, Susan Z.
PUB. DATE
October 2003
SOURCE
Analytical & Bioanalytical Chemistry;Oct2003, Vol. 377 Issue 4, p666
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
meso-2,3-Dimercaptosuccinic acid (meso-DMSA) is an effective chelating agent for the treatment of lead poisoning. We have developed a capillary electrophoresis (CE) method to monitor the urinary excretion of meso-DMSA in human beings. The urine sample was directly injected for analysis in CE without the requirement of solid-phase extraction (SPE). The meso-DMSA was detected in 20 mM borate buffer (pH 8.3) using a 60-cm length bare fused-silica capillary (75-μm ID, 52.5-cm effective length). The meso-DMSA can be extensively biotransformed during metabolism, and no meso-DMSA in urine samples was found in our studies. Any metabolized meso-DMSA can be successfully converted to free meso-DMSA by chemical reduction with dithiothreitol (DTT). In addition, samples were also treated with ethylenediaminetetraacetic acid (EDTA) to transchelate any meso-DMSA that is coordinated with metal ions present in the urine samples. The total amount of meso-DMSA present as these chemical forms was quantified after chemical reduction and addition of EDTA. The detection limit of meso-DMSA was about 50 μM, the RSD of peak area and migration time of meso-DMSA were 4–8% and less than 1%, respectively.
ACCESSION #
15124739

 

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