Array biosensor for detection of toxins

Ligler, Frances S.; Taitt, Chris Rowe; Shriver-Lake, Lisa C.; Sapsford, Kim E.; Shubin, Yura; Golden, Joel P.
October 2003
Analytical & Bioanalytical Chemistry;Oct2003, Vol. 377 Issue 3, p469
Academic Journal
The array biosensor is capable of detecting multiple targets rapidly and simultaneously on the surface of a single waveguide. Sandwich and competitive fluoroimmunoassays have been developed to detect high and low molecular weight toxins, respectively, in complex samples. Recognition molecules (usually antibodies) were first immobilized in specific locations on the waveguide and the resultant patterned array was used to interrogate up to 12 different samples for the presence of multiple different analytes. Upon binding of a fluorescent analyte or fluorescent immunocomplex, the pattern of fluorescent spots was detected using a CCD camera. Automated image analysis was used to determine a mean fluorescence value for each assay spot and to subtract the local background signal. The location of the spot and its mean fluorescence value were used to determine the toxin identity and concentration. Toxins were measured in clinical fluids, environmental samples and foods, with minimal sample preparation. Results are shown for rapid analyses of staphylococcal enterotoxin B, ricin, cholera toxin, botulinum toxoids, trinitrotoluene, and the mycotoxin fumonisin. Toxins were detected at levels as low as 0.5 ng mL-1.


Related Articles

  • A Bioanalytical Platform for Simultaneous Detection and Quantification of Biological Toxins. Weingart, Oliver G.; Hui Gao; Crevoisier, François; Heitger, Friedrich; Avondet, Marc-Adré; Sigrist, Hans // Sensors (14248220);2012, Vol. 12 Issue 2, p2324 

    The article discusses a study on a bioanalytical platform for the detection and quantification of biological toxins. The platform includes the specificity of covalently immobilized capture probes and an instrumentation and immuno-based microarray analytics. The study showed the efficiency of...

  • Cholera toxin subunit B detection in microfluidic devices. Bunyakul, Natinan; Edwards, Katie; Promptmas, Chamras; Baeumner, Antje // Analytical & Bioanalytical Chemistry;Jan2009, Vol. 393 Issue 1, p177 

    Fluorescence and electrochemical microfluidic biosensors were developed for the detection of cholera toxin subunit B (CTB) as a model analyte. The microfluidic devices were made from polydimethylsiloxane (PDMS) using soft lithography from silicon templates. The polymer channels were sealed with...

  • Knowledge-based design of reagentless fluorescent biosensors from a designed ankyrin repeat protein. Brient-Litzler, Elodie; Plückthun, Andreas; Bedouelle, Hugues // PEDS: Protein Engineering, Design & Selection;Apr2010, Vol. 23 Issue 4, p229 

    Designed ankyrin repeat proteins (DARPins) can be selected from combinatorial libraries to bind any target antigen. They show high levels of recombinant expression, solubility and stability, and contain no cysteine residue. The possibility of obtaining, from any DARPin and at high yields,...

  • Antibody characterization and immunoassays for palytoxin using an SPR biosensor. Yakes, Betsy Jean; DeGrasse, Stacey L.; Poli, Mark; Deeds, Jonathan R. // Analytical & Bioanalytical Chemistry;Oct2011, Vol. 400 Issue 9, p2865 

    Palytoxin (PLTX), a polyether marine toxin originally isolated from the zoanthid Palythoa toxica, is one of the most toxic non-protein substances known. Fatal poisonings have been linked to ingestion of PLTX-contaminated seafood, and effects in humans have been associated with dermal and...

  • Fluorescence-labelled antigen-binding fragments (Fab) from monoclonal antibody 5F12 detect human erythropoietin in immunoaffinity capillary electrophoresis. Bornemann, Claus; Burggraef, Tilman; heimbüchel, Günter; Hanisch, Franz-Georg; Winkels, Sandra // Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 7, p1074 

    A faster and more convenient method is required for the detection of recombinant erythropoietin (Epo) in human body fluids. In the present study we wanted to elucidate the principal suitability of immunoaffinity capillary electrophoresis (CE) in this respect. CE offers itself as a high-speed,...

  • Brighter aptamers for biosensing. DeWitt, Natalie // Nature Biotechnology;Dec2000, Vol. 18 Issue 12, p1233 

    Cites a study by researcher Sulay Jhaveri and colleagues on the development of an aptamer with signal transduction. In vitro selection of fluorescently labeled aptamers.

  • 2002 W.A.E. McBryde Award Lecture � Affinity recognition, capillary electrophoresis, and laser-induced fluorescence polarization for ultrasensitive bioanalysis. Le, X. Chris; Pavski, Victor; Wang, Hailin // Canadian Journal of Chemistry;Mar2005, Vol. 83 Issue 3, p185 

    The combination of affinity recognition, capillary electrophoresis (CE), laser-induced fluorescence (LIF), and fluorescence polarization for the ultrasensitive determination of compounds of biological interest is described. Competitive immunoassays using CE�LIF eliminate the need for...

  • Chiral discrimination using an immunosensor. Hofstetter, Oliver; Hofstetter, Heike; Wilchek, Meir; Schurig, Volker; Green, Bernard S. // Nature Biotechnology;Apr99, Vol. 17 Issue 4, p371 

    Based on the stereoselectivity of immunoglobulins, we have developed a new chiral sensor for the detection of low-molecular-weight analytes. Using surface plasmon resonance detection, enantiomers of free, underivatizedα-amino acids can be monitored in a competitive assay by their interaction...

  • Antibody-free lanthanide-based fluorescent probe for determination of protein tyrosine kinase and phosphatase activities. Appelblom, Heidi; Sipponen, Aura; Valanne, Antti; Soukka, Tero; Lövgren, Timo; Niemelä, Pauliina // Microchimica Acta;Feb2011, Vol. 172 Issue 1/2, p25 

    single-labeled peptide probe for measuring peptide phosphorylation status was developed by using a phosphate sensitive terbium chelate. The activity of Abl protein tyrosine kinase and T-cell protein Tyrosine phosphatase (TC PTP) was monitored in real time. To study the probe design in detail,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics