Precision studies using the ABI Prism 3100 Genetic Analyzer for forensic DNA analysis

Sgueglia, Joanne B.; Geiger, Stephana; Davis, Jeffrey
August 2003
Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 8, p1247
Academic Journal
Precision studies using the ABI Prism 3100 Genetic Analyzer have been conducted by performing multiple runs of the AmpFâ„“STR Identifiler allelic ladder, 9947A kit positive control DNA and the 250-base-pair fragment from the internal size standard (GeneScan LIZ-500). Intra-run and inter-run precision data demonstrates the sizing reproducibility of the 3100 instrument for forensic applications. This precision data is utilized to assist in making allele assignments for outlier and variant alleles (i.e., 'off-ladder' alleles). It also provides a means of addressing systematic variations due to temperature fluctuations, polymer (POP-4) or capillary effects, and associated troubleshooting efforts.


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