Quantification of DNA in forensic samples

Nicklas, Janice A.; Buel, Eric
August 2003
Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 8, p1160
Academic Journal
Quantification of DNA in a forensic sample is of major importance for proper DNA amplification and STR profiling. Several methods have been developed to quantify DNA, from basic UV spectrometry, through gel-based techniques, to dye staining, blotting techniques, and, very recently, DNA amplification methods (polymerase chain reaction, PCR). Early techniques simply measured total DNA, but newer techniques can specifically measure human DNA while excluding non-human DNA (foodstuff, animal, or bacterial contamination). These newer assays can be faster and less expensive than traditional methods, making them ideal for the busy forensic laboratory. This paper reviews classic and newer quantification techniques and presents methods recently developed by the authors on the basis of PCR of Alu sequences.


Related Articles

  • Large fragment Bst DNA polymerase for whole genome amplification of DNA from formalin-fixed paraffin-embedded tissues. Aviel-Ronen, Sarit; Qi Zhu, Chang; Coe, Bradley P; Liu, Ni; Watson, Spencer K; Lam, Wan L; Tsao, Ming Sound // BMC Genomics;2006, Vol. 7, p1 

    Background: Formalin-fixed paraffin-embedded (FFPE) tissues represent the largest source of archival biological material available for genomic studies of human cancer. Therefore, it is desirable to develop methods that enable whole genome amplification (WGA) using DNA extracted from FFPE...

  • Quantification of damage in DNA recovered from highly degraded samples -- a case study on DNA in faeces. Deagle, Bruce E.; Eveson, J. Paige; Jarman, Simon N. // Frontiers in Zoology;2006, Vol. 3, p11 

    Background: Poorly preserved biological tissues have become an important source of DNA for a wide range of zoological studies. Measuring the quality of DNA obtained from these samples is often desired; however, there are no widely used techniques available for quantifying damage in highly...

  • Interaction of Replication Protein A and Flap Endonuclease 1 with DNA Duplexes Containing a Nick or a Flap. Khlimankov, D. Yu.; Rechkunova, N. I.; Khodyreva, S. N.; Petruseva, I. O.; Nazarkina, Zh. K.; Belousova, E. A.; Lavrik, O. I. // Molecular Biology;Nov2002, Vol. 36 Issue 6, p849 

    Nicks and flaps are intermediates in various processes of DNA metabolism, including replication and repair. Photoaffinity modification was employed in studying the interaction of the replication protein A (RPA) and flap endonuclease 1 (FEN-1) with DNA duplexes similar to structures arising...

  • Molecular confirmation of a new herpesvirus from catfish ( Ameiurus melas) by testing the performance of a novel PCR method, designed to target the DNA polymerase gene of alloherpesviruses. Doszpoly, Andor; Kovács, Endre R.; Bovo, Giuseppe; LaPatra, Scott E.; Harrach, Balázs; Benkő, Mária // Archives of Virology;Nov2008, Vol. 153 Issue 11, p2123 

    A PCR method with consensus degenerate primers was developed for the detection of herpesviruses (HVs) of anamnia. Compared to previously published PCRs, targeting the DNA polymerase gene of fish HVs, the size of PCR products was more than tripled. Although broad applicability of the method could...

  • Interspecies Translation of Disease Networks Increases Robustness and Predictive Accuracy. Anvar, Seyed Yahya; Tucker, Allan; Vinciotti, Veronica; Venema, Andrea; van Ommen, Gert-Jan B.; van der Maarel, Silvere M.; Raz, Vered; 't Hoen, Peter A. C. // PLoS Computational Biology;Nov2011, Vol. 7 Issue 11, Special section p1 

    Gene regulatory networks give important insights into the mechanisms underlying physiology and pathophysiology. The derivation of gene regulatory networks from high-throughput expression data via machine learning strategies is problematic as the reliability of these models is often compromised...

  • GO ON GREEN. Netterwald, James // Drug Discovery & Development;Feb2007, Vol. 10 Issue 2, pG1 

    The article provides information concerning on the validation of microarray results in the U.S. One way to validate microarray results is by using probe-based quantitative polymerase chain reaction (qPCR). This method has a greater specificity due to its use of two primers and an oligonucleotide...

  • Detection of Recombinant Products during PCR Amplification of DNA Containing Direct Alu Repeats. Shibalev, D. V.; Voronov, A. S.; Bashkirov, V. N.; Kupriyanova, N. S.; Ryskov, A. P. // Doklady Biochemistry & Biophysics;Jan/Feb2003, Vol. 388 Issue 1-6, p55 

    Presents information on a study which examined the detection of recombinant products during polymerase chain reaction (PCR) amplification of DNA containing direct alu repeats. Recombinant products formed during PCR amplification; Study methods; Results.

  • Quantitative polymerase chain reaction analysis by deconvolution of internal standard. Hirakawa, Yasuko; Medh, Rheem D.; Metzenberg, Stan // BMC Molecular Biology;2010, Vol. 11, p30 

    Background: Quantitative Polymerase Chain Reaction (qPCR) is a collection of methods for estimating the number of copies of a specific DNA template in a sample, but one that is not universally accepted because it can lead to highly inaccurate (albeit precise) results. The fundamental problem is...

  • Electrical DNA-chip-based identification of different species of the genus Kitasatospora. Möller, Robert; Schüler, Thomas; Günther, Sebastian; Carlsohn, Marc René; Munder, Thomas; Fritzsche, Wolfgang // Applied Microbiology & Biotechnology;Jan2008, Vol. 77 Issue 5, p1181 

    The identification of different Kitasatospora strains has been shown with a DNA-chip based on an electrical readout scheme. The 16S-23S rDNA internal transcribed spacer region of these Actinomycetes was used for identification. Two different capture probes per strain were immobilized on the...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics