TITLE

Splitless on-line coupling of capillary high-performance liquid chromatography, capillary electrochromatography and pressurized capillary electrochromatography with nuclear magnetic resonance spectroscopy

AUTHOR(S)
Rapp, Erdmann; Jakob, Andreas; Schefer, Alexandre Bezerra; Bayer, Ernst; Albert, Klaus
PUB. DATE
August 2003
SOURCE
Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 7, p1053
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A mixture of unsaturated fatty acid methyl esters was separated with a new splitless capillary set-up. With the employed apparatus configuration different capillary separation techniques such as capillary high-performance liquid chromatography (cHPLC), capillary electrochromatography (CEC) and pressurized capillary electrochromatography (pCEC) could be applied. The detection and identification of the sample compounds were accomplished by hyphenating these capillary separation techniques with nuclear magnetic resonance (NMR) spectroscopy using a novel configuration of the detection capillary set-up. Using modified electrokinetically driven separation techniques, the electric field was applied solely across the separation column. With this improved interface for capillary liquid chromatography-NMR on-line coupling, the stereochemical assignment of the cis and trans configuration of unsaturated fatty acids could be easily accomplished. Finally, the results of cHPLC-NMR, CEC-NMR and pCEC-NMR coupling experiments were compared.
ACCESSION #
15124645

 

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