TITLE

Splitless on-line coupling of capillary high-performance liquid chromatography, capillary electrochromatography and pressurized capillary electrochromatography with nuclear magnetic resonance spectroscopy

AUTHOR(S)
Rapp, Erdmann; Jakob, Andreas; Schefer, Alexandre Bezerra; Bayer, Ernst; Albert, Klaus
PUB. DATE
August 2003
SOURCE
Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 7, p1053
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A mixture of unsaturated fatty acid methyl esters was separated with a new splitless capillary set-up. With the employed apparatus configuration different capillary separation techniques such as capillary high-performance liquid chromatography (cHPLC), capillary electrochromatography (CEC) and pressurized capillary electrochromatography (pCEC) could be applied. The detection and identification of the sample compounds were accomplished by hyphenating these capillary separation techniques with nuclear magnetic resonance (NMR) spectroscopy using a novel configuration of the detection capillary set-up. Using modified electrokinetically driven separation techniques, the electric field was applied solely across the separation column. With this improved interface for capillary liquid chromatography-NMR on-line coupling, the stereochemical assignment of the cis and trans configuration of unsaturated fatty acids could be easily accomplished. Finally, the results of cHPLC-NMR, CEC-NMR and pCEC-NMR coupling experiments were compared.
ACCESSION #
15124645

 

Related Articles

  • Isolation and Characterization of Fatty Acids Ester from the Thai Sea Gorgonian, Junceella sp. Pedpradab, Patchara; Molex, Wallapa; Suwanborirux, Khanit // International Proceedings of Chemical, Biological & Environmenta;2012, Vol. 38, p119 

    This research has the main aim to search for chemical constituents from the marine organisms in the Andaman Sea. A various chromatographic methods were used to isolate the constituted fatty acids from a white gorgonian, Juncella sp. which was collected around Lanta Island, Krabi Province,...

  • When Peaks Collide (Part 3). Hinshaw, John V. // LC-GC Europe;Nov2010, Vol. 23 Issue 10, p531 

    The article discusses a study which evaluates the quantitative area measurement of two tailing peaks of chromatographic devices. It reveals the significance of peak discrimination factor in indicating how well a specified separation discriminates adjacent peaks from each other and how it...

  • Developments in Preperative-Scale Chromatography Columns and Accessories. Majors, Ronald F. // LC-GC Europe;Dec2004, Vol. 17 Issue 12, p630 

    In this month's "Column Watch," the author focuses on some of the latest developments in preparative-scale columns, bulk packing materials, and column hardware designs. Silica, polymeric, and other packings are discussed along with the newest monolithic columns, which can offer advantages over...

  • SUB-MICRON MACHINING FORMS HIGH PERFORMANCE ON-WAFER CHROMATOGRAPH.  // Electronics World;Apr2008, Vol. 114 Issue 1864, p6 

    The article reports on the on-wafer liquid phase chromatograph developed by sub-micron machining. The chromatograph provides a five to 10-fold increase in the speed of analysis and has 56 separation channels. According to the article, the chromatograph can be used to separate and identify...

  • Optimizing Detector Set-up and Operating Conditions. Hinshaw, John V. // LC-GC Europe;Mar2009, Vol. 22 Issue 3, p137 

    Detectors are another link in the chain of signals that a chromatographic analysis generates. After separation, a detector transduces the chemical signals of eluted analytes to an electrical signal that is then recorded and measured. The identities and amounts of analytes are determined from...

  • The Importance of the Nebulizer Design on the Performance of an Evaporative Light-Scattering Detector.  // LC-GC North America;Jun2004 Supplement, Vol. 22, p74 

    Investigates the importance of the nebulizer design in the use of evaporative light-scattering detector. Cause of the degradation of the resolution in the chromatogram; Result of the optimization of the nebulizer design; Quantitation of the compounds of interest.

  • A Review of Chromatographic Characterization Techniques for Biodiesel and Biodiesel Blends. Pauls // Journal of Chromatographic Science;May2011, Vol. 49 Issue 5, p384 

    This review surveys chromatographic technology that has been applied to the characterization of biodiesel and its blends. Typically, biodiesel consists of fatty acid methyl esters produced by transesterification of plant or animal derived triacylglycerols. Primary attention is given to the...

  • Identification of Bacillus anthracis from Culture using Gas Chromatographic Analysis of Fatty Acid Methyl Esters. Sasser, Myron; Kunitsky, Craig; Jackoway, Gary; Ezzell, John W.; Teska, Jeffrey D.; Harper, Bruce; Parker, Stephen // Journal of AOAC International;Jan/Feb2005, Vol. 88 Issue 1, p178 

    Cites a study on the identification of Bacillus anthracis from culture using gas chromatographic analysis of fatty acid methyl esters. Discussion on the procedure used in the extraction of fatty acids; Basis of bacterial identification through the use of MIDI method; Determination whether the...

  • The Integration of Microextraction Packed Sorbent (MEPS) into Multidimensional Stratagies. Dawes, Peter; Dawes, Ern; DiFeo, Dan; Hibbert, Roy; Wynne, Paul // LC-GC North America;Feb2009 Supplement, Vol. 27, p53 

    The article offers information on the chromatographic analysis of complex fatty acid methyl esters (FAME) samples using the microextraction packed sorbent (MEPS) format. The MEPS method is based on either strong retention or full elution of particular groups. The MEPS method generates a three...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics