Screen-printed multienzyme arrays for use in amperometric batch and flow systems

Sapelnikova, S.; Dock, E.; Solná, R.; Skládal, P.; Ruzgas, T.; Emnéus, J.
August 2003
Analytical & Bioanalytical Chemistry;Aug2003, Vol. 376 Issue 7, p1098
Academic Journal
Screen-printing technology for electrode fabrication enables construction of amperometric devices suitable for combination of several enzyme electrodes. To develop a biosensor array for characterisation of wastewaters, tyrosinase and horseradish peroxidase (HRP) or cholinesterase-modified electrodes were combined on the same array. The behaviour of the tyrosinase-modified electrode in the presence of hydrogen peroxide (required co-substrate for the HRP-modified electrode) and acetylthiocholine chloride (required co-substrate for cholinesterase) was studied. Performance of bi-enzyme biosensor arrays in the batch mode and in the flow-injection system are discussed.


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