TITLE

Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples

AUTHOR(S)
Li, Bin; Wang, Qiuquan; Yan, Hua; Yang, Limin; Huang, Benli
PUB. DATE
July 2003
SOURCE
Analytical & Bioanalytical Chemistry;Jul2003, Vol. 376 Issue 6, p923
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A novel reversed phase ion-pair micro HPLC system with on-line fluorescence detection has been developed systematically and studied for the determination of cadmium in its bio-available fractions of soil samples. In this system, a micro ODS column of 1.0 mm i.d.×150 mm length and a mobile phase containing 6 mmol L-1 8-hydroxyquinoline 5-sulphonic acid (HQS), 3 mmol L-1 cetyltrimethylammonium bromide (CTMABr), 10 mmol L-1 acetic acid-acetate buffer (pH 5.1) as well as 50% acetonitrile at 50 μL min-1 flow rate were employed to determine cadmium with a 2 μL flow cell through its fluorescence at 518 nm under 338 nm excitation. Furthermore, the composition of Cd-HQS chelate formed on the column was confirmed to be [Cd(HQS)2]2- through a log–log plot method, and then combined with the ion-pair reagent by the electrostatic force under the chromatographic condition proposed. With such a method, the detection limit of cadmium was 8.48 ng mL-1 (3σ) with 1 μL sample injection, and the linear range for the determination of cadmium was 30–800 ng mL-1 (R2=0.992). This method has been successfully applied to determination of cadmium in its bio-available fractions of BCR-483 and soil samples without interference from other coexistent metal ions. The RSD (n=6) was less than 7.3%. The results were in agreement with the indicative value for BCR-483 and those for the soil samples obtained by ICP-MS with a pretreatment of bis(1,1,3,3-tetramethylbutyl) phosphinic acid extraction.
ACCESSION #
15124637

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics