Determination of berberine by measuring the enhanced total internal reflected fluorescence at water/tetrachloromethane interface in the presence of sodium dodecyl benzene sulfonate

Ping Feng; Cheng Zhi Huang; Yuan Fang Li
July 2003
Analytical & Bioanalytical Chemistry;Jul2003, Vol. 376 Issue 6, p868
Academic Journal
A highly sensitive method for determination of berberine is proposed based on the measurements of total internal reflected fluorescence (TIRF) at water/ tetrachloromethane (H2O/CCl4) interface. In the pH range of 2.6–5.7, the co-adsorption of the berberine with the anionic surfactants such as sodium dodecyl benzene sulfonate (SDBS), sodium dodecylsulfonate (SDS), and sodium lauryl sulfate (SLS) occurs at the H2O/CCl4 interface, resulting in greatly enhanced TIRF signal characterized by the emission at 526 nm when excited with a 351 nm light beam. The enhanced TIRF intensity is in proportion to the berberine concentration in the range 0.2–10.0×10-7 mol L-1. The limit of detection is 1.7×10-9 mol L-1 (3σ). It was found that ions such as Ca(II), Cu(II), Fe(III), Cd(II), Mg(II), Zn(II), Pb(II), and Al(III) can be allowed larger than 1.0×10-4 mol L-1. Meanwhile, the organic compounds such as vitamin B, saccharine, and amino acid do not display any effect for the present TIRF method even if they are larger than 1.0×10-2 mol L-1in high concentration levels (larger than 1.0×10-5 mol L-1). The results of determination for synthetic samples were agreement with the desired values, and the ones for tablets were identical with those obtained according to the method of Chinese Pharmacopoeia.


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