TITLE

Isolation and determination of p-hydroxybenzoylcholine in traditional Chinese medicine Semen sinapis Albae

AUTHOR(S)
Lifang Liu; Tao Liu; Genxi Li; Qiang Wang; Tzibun Ng
PUB. DATE
July 2003
SOURCE
Analytical & Bioanalytical Chemistry;Jul2003, Vol. 376 Issue 6, p854
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A special component is isolated from Semen sinapis Albae (white mustard seed), a traditional Chinese medicine. According to the physical and chemical investigation and spectroscopic identification, this component can be known as p-hydroxybenzoylcholine bisulfate, a choline base. This component in the drug is also determined by RP-HPLC. A reversed-phase C18 column is used to separate the p-hydroxybenzoylcholine with an eluent of methanol–0.05 mol/L monopotassium phosphate solution (30:70) (adjusted by phosphoric acid to pH 3.6) at the flow rate of 0.5 mL/min. Detection is carried out with a UV detector operated at 285 nm, and the column temperature is 25 °C. It reveals that there is 0.021% (w/w) of p-hydroxybenzoylcholine bisulfate in Semen sinapis Albae and 0.037% (w/w) in stir-baked Semen sinapis Albae.
ACCESSION #
15124614

 

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