TITLE

Determination of methylparaben, propylparaben, triamcinolone acetonide and its degradation product in a topical cream by RP-HPLC

AUTHOR(S)
Matysová, L.; Hájková, R.; Šícha, J.; Solich, P.
PUB. DATE
June 2003
SOURCE
Analytical & Bioanalytical Chemistry;Jun2003, Vol. 376 Issue 4, p440
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A novel reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone (occurring in formulation after long-term stability tests) and two preservatives presented in the cream—methylparaben and propylparaben, using hydrocortisone as an internal standard. The chromatographic separation was performed on a Supelco Discovery C18 column; the mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (40:60 v/v). The analysis time was less than 9 min, at a flow rate of 0.6 mL min-1 and detection at 240 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolon cream 0.1%.
ACCESSION #
15124595

 

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