TITLE

Scanning-force techniques to monitor time-dependent changes in topography and adhesion force of proteins on surfaces

AUTHOR(S)
Mondon, M.; Berger, S.; Ziegler, C.
PUB. DATE
April 2003
SOURCE
Analytical & Bioanalytical Chemistry;Apr2003, Vol. 375 Issue 7, p849
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Scanning-force microscopy (SFM) investigations were conducted to probe the influences of the interactions of proteins with surfaces relevant in medicine. These interactions are an important feature in the area of biofilm formation. The adsorption of proteins leads to changes in topography, which was monitored for the build up of protein layers of hen egg-white lysozyme and bovine serum albumin (BSA) on mica in real time in phosphate-buffered aqueous solution over a time period of 10 min. Phase imaging was additionally applied to compare material contrasts and to evaluate this method for further application in this field. The adhesion forces that develop on a time scale below 20 s between a protein-modified SFM tip and titanium surfaces (TiO2, TiAl6V4 and TiAl6Nb7) were investigated. The influences of the parameters loading force and interaction time between the protein and the surface were monitored as well as the influence of protein structure. The interaction time dependency of the adhesion force could be described with a kinetic model of two consecutive first-order reactions. For the maximal adhesion force a correlation to the ratio of the amino acids cysteine, proline and glycine has been proposed.
ACCESSION #
15124553

 

Related Articles

  • Ab initio-based exciton model of amide I vibrations in peptides: Definition, conformational dependence, and transferability. Gorbunov, Roman D.; Kosov, Daniil S.; Stock, Gerhard // Journal of Chemical Physics;6/8/2005, Vol. 122 Issue 22, p224904 

    Various aspects of the ab initio-based parametrization of an exciton model of amide I vibrations in peptides are discussed. Adopting “glycine dipeptide” (Ac-Gly-NHCH3) as a simple building-block model that describes the vibrational interaction between two peptide units, we perform...

  • Analysis of Recognition Elements for Mitochondrial Processing Peptidase Using Artificial Amino Acids: Roles of the Intervening Portion and Proximal Arginine1. Moriwaki, Kaori; Ogishima, Tadashi; Ito, Akio // Journal of Biochemistry;1999, Vol. 126 Issue 5, p874 

    We recently demonstrated, using synthetic peptides modeled on the extension peptide of malate dehydrogenase, that amino acid residues present at the proximal and distal positions relative to the cleavage site are critical determinants for the recognition of substrates by mitochondrial processing...

  • Tikhonov adaptively regularized gamma variate fitting to assess plasma clearance of inert renal markers. Wesolowski, Carl; Puetter, Richard; Ling, Lin; Babyn, Paul // Journal of Pharmacokinetics & Pharmacodynamics;Oct2010, Vol. 37 Issue 5, p435 

    The Tk-GV model fits Gamma Variates (GV) to data by Tikhonov regularization (Tk) with shrinkage constant, λ, chosen to minimize the relative error in plasma clearance, CL (ml/min). Using Yb-DTPA and Tc-DTPA ( n = 46, 8-9 samples, 5-240 min) bolus-dilution curves, results were obtained for fit...

  • Altered drug binding to serum proteins in pregnant women: therapeutic relevance. Perucca, E.; Ruprah, M.; Richens, A. // Journal of the Royal Society of Medicine;Jun1981, Vol. 74 Issue 6, p422 

    The binding of diazepam, phenytoin and valproic acid to serum proteins in vitro has been compared in pregnant women of different gestational ages and in controls. The unbound fraction of each of the three drugs was elevated during pregnancy (particularly during the last 8 weeks) probably due, at...

  • Extracellular yeast-lytic enzyme of the bacterium Lysobacter sp. XL 1. Stepnaya, O.; Tsfasman, I. M.; Chaika, I. A.; Muranova, T. A.; Kulaev, I. S. // Biochemistry (00062979);Mar2008, Vol. 73 Issue 3, p310 

    An enzyme exhibiting yeast-lytic activity has been isolated from the culture liquid of the bacterium Lysobacter sp. XL. 1. The optimal conditions for the hydrolysis of Saccharomyces cerevisiae cells by the enzyme have been established: 0.15 M sodium acetate buffer, pH 6.0, 50°C. The...

  • Membrane selection and muscle protein catabolism. Gutierrez, Alberto; Alvestrand, Anders; Bergström, Jonas // Kidney International Supplement;Oct1992, Issue 38, pS-86 

    Investigates the effects of blood membrane on muscle protein catabolism in fasting subjects. Application of hemodialysis procedure as a strong catabolic stimulus for the increase protein requirement in patient; Details on the analysis of free amino acids in healthy participants; Findings on the...

  • albumin. Peters, Michael // BMA A-Z Family Medical Encyclopedia;2004, p26 

    An encyclopedia entry for "albumin" is presented. It refers to the most common protein in the blood plasma that is produced in the liver from amino acids obtained from digested protein. The said protein has various responsibilities, one of which is to keep substances, such as calcium and...

  • The “Invisible Proteome”: How to Capture the Low-Abundance Proteins Via Combinatorial Ligand Libraries. Boschetti, Egisto; Monsarrat, Bernard; Righetti, Pier Giorgio // Current Proteomics;Dec2007, Vol. 4 Issue 4, p198 

    Despite recent advances in pre-fractionation procedures and depletion methods via immuno-subtraction protocols of the most abundant species, the “low-abundance” proteome remains largely undetected. We report here a novel technology, called ProteoMiner, for bringing to the limelight...

  • Structure of C3b reveals conformational changes that underlie complement activity. Janssen, Bert J. C.; Christodoulidou, Agni; McCarthy, Andrew; Lambris, John D.; Gros, Piet // Nature;11/9/2006, Vol. 444 Issue 7116, p213 

    Resistance to infection and clearance of cell debris in mammals depend on the activation of the complement system, which is an important component of innate and adaptive immunity. Central to the complement system is the activated form of C3, called C3b, which attaches covalently to target...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics