Determination of low molecular weight organic acids in soil, plants, and water by capillary zone electrophoresis

Ying-Hui Li; Bi-Xia Huang; Xiao-Quan Shan
March 2003
Analytical & Bioanalytical Chemistry;Mar2003, Vol. 375 Issue 6, p775
Academic Journal
Determination of low molecular weight organic acids in soils and plants by capillary zone electrophoresis was accomplished using a phthalate buffer and indirect UV detection mode. The influence of some crucial parameters, such as pH, buffer concentration and surfactant were investigated. A good separation of seven organic acids was achieved within 5 min using an electrolyte containing 15 mmol L-1 potassium hydrogen phthalate, 0.5 mmol L-1 myristyltrimethylammonium bromide (MTAB), and 5% methanol (MeOH) (v/v) at pH 5.60, separation voltage -20 kV, and temperature 25 °C. The relative standard deviation (n=5) of the method was found to be in range 0.18–0.56% for migration time and 3.2–4.8% for peak area. The limit of detection ranged between 0.5 µmol L-1 to 6 µmol L-1 at a signal-to-noise ratio of 3. The recovery of standard organic acids added to real samples ranged from 87 to 119%. This method was simple, rapid and reproducible, and could be applied to the simultaneous determination of organic acids in environmental samples.


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