Determination of Serum Albumin in Blood by Constant-Current Coulometry Using Electrochemically Generated Oxidizers

Ziyatdinova, G. K.; Budnikov, G. K.; Pogorel'tsev, V. I.
July 2004
Journal of Analytical Chemistry;Jul2004, Vol. 59 Issue 7, p659
Academic Journal
A method is developed for the determination of human serum albumin by constant-current coulometry using electrochemically generated oxidizers. It is found that human serum albumin interacts with electrochemically generated bromine and iodine at a ratio of 1 : 73 and 1 : 64, respectively. Milligrams of human serum albumin were determined in model solutions with RSD = 1–2%. A procedure is proposed for the determination of serum albumin in human blood.


Related Articles

  • HSA.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1096 

    An encyclopedia entry for the medical acronym "HSA," which stands for human serum albumin, is presented.

  • Influence of ultrasound irradiation on the adsorption of bovine serum albumin on copper. Pinto, Edilson M.; Soares, David M.; Brett, Christopher M. A. // Journal of Applied Electrochemistry;Nov2007, Vol. 37 Issue 11, p1367 

    The effect of irradiation by power ultrasound on the adsorption of proteins on copper has been investigated, using bovine serum albumin (BSA) as a model protein in pH 7 phosphate buffer solution. Open circuit potential measurements, cyclic voltammetry and electrochemical impedance spectroscopy...

  • Blood from Grains. Breindl, Anette // BioWorld Today;11/7/2011, Vol. 22 Issue 216, Special section p2 

    The article reports that scientists from Wuhan University in China have produced recombinant human serum albumin from rice.

  • Synchronous Fluorescence Determination of Protein with Functional Organic Nanoparticles. Tingting Xia; Lun Wang; Guirong Bian; Ling Dong; Shi Hong // Microchimica Acta;Jun2006, Vol. 154 Issue 3/4, p309 

    A new method for detecting protein using a synchronous fluorescence scan technique with a novel composite organic nanoparticle has been developed. The novel functional organic nanoparticle, dodecyl benzene sulfonic acid sodium salt (DBSS)-capped nano-anthracene, is easily prepared by...

  • Assessment of hypoproteinaemic oedema: a simple physical sign. Henry, J.A.; Altmann, P. // British Medical Journal;4/8/1978, Vol. 1 Issue 6117, p890 

    Assessment of hypoproteinaemic oedema: a simple physical sign It is well known that hypoproteinaemia can cause pitting oedema. We have found that hypoproteinaemic oedema pits and recovers differently from other types of oedema but can find no reference to this in reports or texts on physical...

  • Bromophenol Blue Binding as a Probe to Study Urea and Guanidine Hydrochloride Denaturation of Bovine Serum Albumin. Halim, Adyani Azizah Abd.; Kadir, Habsah Abdul; Tayyab, Saad // Journal of Biochemistry;Jul2008, Vol. 144 Issue 1, p33 

    Urea and guanidine hydrochloride (GdnHCl) denaturation of bovine serum albumin (BSA) were investigated using bromophenol blue (BPB) binding as a probe. Addition of BPB to BSA produced an absorption difference spectrum in the wavelength range, 525–675 nm with a minimum at 587 nm and a...

  • Influences of haemodialysis on the binding sites of human serum albumin: possibility of an efficacious administration plan using binding inhibition. Toyotaka Nishio; Norito Takamura; Ryuuichi Nishii; Jin Tokunaga; Mitsuyoshi Yoshimoto; Keiichi Kawai // Nephrology Dialysis Transplantation;Jul2008, Vol. 23 Issue 7, p2304 

    Background. We have studied the possibility that low-dose treatment utilizing the inhibition that may occur between two drugs at the same site of human serum albumin (HSA) improves the pharmacological effects. The purpose is to elucidate the differences in the binding capacities of sites I and...

  • Synchronous fluorescence determination and molecular modeling of 5-Aminosalicylic acid (5-ASA) interacted with human serum albumin. Feng-ling Cui; Li-xia Qin; Fang Li; Hong-xia Luo // Journal of Molecular Modeling;Dec2008, Vol. 14 Issue 12, p1111 

    Abstract  In this paper, we proposed a new method for the determination of either human serum albumin (HSA) or 5-Aminosalicylic acid (5-ASA) by synchronous fluorescence spectra and examined the interaction between them using the molecular modeling method under simulative...

  • Interaction and diffusion of gold nanoparticles in bovine serum albumin solutions. Kohli, Indermeet; Alam, Sharmine; Patel, Bhavdeep; Mukhopadhyay, Ashis // Applied Physics Letters;5/20/2013, Vol. 102 Issue 20, p203705 

    We studied the interactions of small sized, radius of 2.5-10 nm, gold nanoparticles (AuNPs) with bovine serum albumin (BSA). Fluctuation correlation spectroscopy (FCS) was used to monitor the changes in the Brownian diffusion of these NPs. Our results indicate a BSA monolayer formation at the NP...


Other Topics