Molecular markers linked to the apple scab resistance geneVbjderived fromMalus baccata jackii

Gygax, M.; Gianfranceschi, L.; Liebhard, R.; Kellerhals, M.; Gessler, C.; Patocchi, A.
November 2004
Theoretical & Applied Genetics;Nov2004, Vol. 109 Issue 8, p1702
Academic Journal
Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungusVenturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance geneVbjoriginating from the crab appleMalus baccata jackii. Using bulk segregant analysis, three RAPD markers linked toVbjwere first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be co-dominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype-phenotype incongruence (GPI plants) plants, a linkage map was calculated.Vbjmapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6 cM from CH05e03 and at 3.9 cM from T6-SCAR. Without the removal of the GPI plants,Vbjwas placed 15 cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated.


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