TITLE

Teflonâ„¢-coated silicon apertures for supported lipid bilayer membranes

AUTHOR(S)
Wilk, S. J.; Goryll, M.; Laws, G. M.; Goodnick, S. M.; Thornton, T. J.; Saraniti, M.; Tang, J.; Eisenberg, R. S.
PUB. DATE
October 2004
SOURCE
Applied Physics Letters;10/11/2004, Vol. 85 Issue 15, p3307
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We present a method for microfabricating apertures in a silicon substrate using well-known cleanroom technologies resulting in highly reproducible giga-seal resistance bilayer formations. Using a plasma etcher, 150 μm apertures have been etched through a silicon wafer. Teflon™ has been chemically vapor deposited so that the surface resembles bulk Teflon and is hydrophobic. After fabrication, reproducible high resistance bilayers were formed and characteristic measurements of a self-inserted single OmpF porin ion channel protein were made.
ACCESSION #
14909432

 

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