Laminin distribution in developing glomerular basement membranes

Abrahamson, Dale R.; St. John, Patricia L.
January 1993
Kidney International;Jan1993, Vol. 43 Issue 1, p73
Academic Journal
The renal glomerular basement membrane (GBM) separates two distinctly different cell layers: the vascular endothelium, and visceral epithelial podocytes. When initial vascularization of the forming glomerulus takes place during nephrogenesis, the early GBM forms by fusion of a dual basement membrane between endothelial cells and podocytes. As glomerular capillary loops blossom, newly synthesized basement membrane segments derived from podocytes are then inserted or spliced into the fused GBM. The molecular processes accounting for either basement membrane fusion or splicing are unresolved. Using monoclonal anti-mouse laminin antibodies (mAbs) against the end of the laminin long arm (5D3), we have shown in adult mice that peripheral loop GBM is only weakly immunoreactive but the mesangial matrix and tubular basement membrane (TBM) is intensely positive. In contrast, mAbs against domains in the center of the laminin cross only label TBMs and mesangial matrices of mature mice and GBMs are negative. Immunofluorescence microscopy of neonatal mouse kidneys showed, however, that anti-laminin mAbs brightly labeled developing GBMs of glomeruli undergoing initial vascularization and capillary loop formation. Post-fusion GBMs of maturing stage glomeruli became unreactive for most anti-laminin mAbs but remained positive for 5D3. Our results therefore show that some GBM laminin epitopes are transiently expressed during glomerular development. These changes in GBM immunoreactivities may reflect proteolytic processing during basement membrane fusion and splicing, or temporally controlled synthesis of different laminin isoforms.


Related Articles

  • Glomerular basement membrane necrosis and crescent organization. Bonsib, Stephen M. // Kidney International;May1988, Vol. 33 Issue 5, p966 

    In order to reveal structural damage to the glomerular basement membranes occurring in crescentic glomerulonephritis and the subsequent connective tissue organization of the crescent, 14 kidney biopsies were treated with detergents to remove cellular components and the tissue remaining examined...

  • Basement Membrane and Human Epidermal Differentiation In Vitro. Guo, Ming; Grinnell, Frederick // Journal of Investigative Dermatology;Sep89, Vol. 93 Issue 3, p372 

    To test the role of basement membrane in differentiation of human epidermis reconstituted on human dermis, we prepared dermis with and without basement membrane and cultured epidermal cells on these two dermal substrata. The absence of basement membrane components was confirmed by...

  • Glomerular endothelial cell development. Abrahamson, Dale R. // Kidney International;Oct1999, Vol. 56 Issue 4, p1597 

    Editorial. Comments on the development of glomerular endothelial cells. Potentiation vascular endothelial growth; Promotion of mitogenesis and migration of selected endothelial progenitor cells; Evaluation of centrifugal patterns of glomerulogenesis.

  • Effect of cytokines on the cytoskeleton of resident glomerular cells. Camussi, Giovanni; Mariano, Filippo; Biancone, Luigi; Montrucchio, Giuseppe; Vercellone, Antonio // Kidney International Supplement;Jan1993, Issue 39, pS-32 

    Resident glomerular cells have complex cytoskeletal organizations relevant to maintaining functional and structural integrity. The ability of cells to change shape, develop coordinate directed movements, replicate and interact with contiguous cells or extracellular matrix depends on cytoskeletal...

  • Role of laminin in endothelial cell recognition and differentiation. Schnaper, H. William; Kleinman, Hynda K.; Grant, Derrick S. // Kidney International;Jan1993, Vol. 43 Issue 1, p20 

    The vascular endothelium normally is maintained in a quiescent state, but under certain conditions it is induced to undergo marked changes in behavior and form new vascular structures. A complex interaction among various growth and differentiation factors and the extracellular milieu regulates...

  • Transgenic Expression of Human LAMA5 Suppresses Murine Lama5 mRNA and Laminin a5 Protein Deposition. Steenhard, Brooke M.; Zelenchuk, Adrian; Stroganova, Larysa; Isom, Kathryn; John, Patricia L. St.; Andrews, Glen K.; Peterson, Kenneth R.; Abrahamson, Dale R. // PLoS ONE;2011, Vol. 6 Issue 9, p1 

    Laminin &agr5 is required for kidney glomerular basement membrane (GBM) assembly, and mice with targeted deletions of the Lama5 gene fail to form glomeruli. As a tool to begin to understand factors regulating the expression of the LAMA5 gene, we generated transgenic mice carrying the human LAMA5...

  • Renal basement membranes by ultrahigh resolution scanning electron microscopy. Hironaka, Kazue; Makino, Hirofumi; Yamasaki, Yasushi; Ota, Zensuke // Kidney International;Feb1993, Vol. 43 Issue 2, p334 

    Three-dimensional ultrastructures of basement membranes of the rat kidney were investigated with an ultrahigh resolution scanning electron microscope (HSEM) equipped with a resolving power of 0.5 nm. All cellular components were extracted from renal cortical tissues by sequential-detergent...

  • Analysis of ultrastructural glomerular basement membrane lesions and podocytes associated with proteinuria and sclerosis in Osborne-Mendel rats with progressive glomerulonephropathy. Yasuno, Kyohei; Kamiie, Junichi; Shirota, Kinji // Journal of Veterinary Science;Jun2013, Vol. 14 Issue 2, p223 

    The renal glomeruli of 12 male Osborne-Mendel (OM) rats 3 to 24 weeks old were examined by electron microscopy. Effacement of podocyte foot processes (FPs) developed at 3 weeks of age and became progressively worse over time. Loss or dislocation of the slit membrane was also found. Vacuoles and...

  • AN ELECTRON MICROSCOPICAL STUDY OF GLOMERULAR CHANGES IN EXPERIMENTAL HYDRONEPHROSIS IN RATS. F. Pak# Poy, R. K.; Robertson, J. S. // Australian Journal of Experimental Biology & Medical Science;Feb1959, Vol. 37 Issue 1, p65 

    Presents an electron microscopical study of glomerular changes in experimental hydronephrosis in rats. Formation of the hyalinized glomerulus by collapse of the capillaries with coalescence of thickened capillary basement membrane and loss of the cellular elements of the glomerulus; Secretion of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics