TITLE

Effect of phosphatidylcholine on lymphatic drainage and fluid loss from the peritoneal cavity of sheep

AUTHOR(S)
Zheng-Yi Yuan; Rodela, Helen; Hay, John B.; Oreopoulos, Dimitrios G.; Johnston, Miles G.
PUB. DATE
August 1994
SOURCE
Kidney International;Aug1994, Vol. 46 Issue 2, p520
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The purpose of this investigation was to test the hypothesis that phosphatidylcholine enhances net ultrafiltration by decreasing lymphatic drainage of the peritoneal cavity. Twelve sheep were used in this study. Six animals received 50 ml/kg intraperitoneal infusions of Diancal® 4.25% (490 mOsm/liter) and six received similar volumes of premixed phosphatidylcholine-Dianeal® (510 mOsm/liter). Labeled albumin (25 μCi 125I-human serum albumin) was added to the dialysate as a lymph flow marker. Lymph drainage of the peritoneal cavity was estimated from the appearance of the intraperitoneally administered tracer in the blood. Net ultrafiltration was significantly enhanced by phosphatidylcholine at each hour up to 6 hours post-infusion, and over this period reached 30.3 ± 3.8 ml/kg in the phosphatidylcholine animals compared to 12.2 ± 2.1 ml/kg in the control group. Phosphatidylcholine treatment decreased the volume removed by lymphatics: by six hours 5.5 ± 1.1 ml/kg in the animals receiving phosphatidylcholine, and 10.3 ± 1.0 ml/kg in the control group was drained as lymph. Fluid toss (estimated from the tracer disappearance from the peritoneal cavity) was slightly less in the phosphatidylcholine-treated animals, averaging 15.8 ± 1.6 in this group versus 16.8 ± 1.7 ml/kg in the control sheep. However, these differences were not significant. Phosphatidylcholine significantly increased transcapillary, ultrafiltration (estimate of volume movement into peritoneal cavity without fluid loss) from 27.6 ± 1.5 ml/kg in the controls to 43.8 ± 3.4 ml/kg in the animals receiving phosphatidylcholine. In summary, the inhibitory effect of phosphatidylcholine on lymph drainage may not contribute significantly to the enhanced net ultrafiltration, since total fluid loss from the peritoneal cavity was approximately the same in phosphatidylcholine-treated and untreated groups. We conclude that the main effect of phosphatidylcholine in sheep is to facilitate the movement of liquid from the blared into the peritoneal cavity.
ACCESSION #
14889511

 

Related Articles

  • Lipoprotein phospholipid composition and LCAT activity in nephrotic and analbuminemic rats. Joles, Jaap A.; Willekes-Koolschijn, Nel; Scheek, Leo M.; Koomans, Hein A.; Rabelink, Ton J.; van Tol, Arie // Kidney International;Jul1994, Vol. 46 Issue 1, p97 

    Albumin is an acceptor of lysophosphatidylcholine (LPC), product of the lecithin:cholesterol acyl transferase (LCAT) reaction, and it has been suggested that low LCAT activity and reduced cholesterol esterification rate in patients with the nephrotic syndrome may be linked to depletion of...

  • Extracellular fluid and its proteins: dehydration, shock, and recovery. Holliday, Malcolm A. // Pediatric Nephrology;1999, Vol. 13 Issue 9, p989 

    This review highlights characteristics of extracellular fluid (ECF) that are often overlooked. ECF has, in addition to plasma and interstitial fluid (ISF) surrounding cells, a third large compartment, the ISF of skin and connective tissue. This acts as a reservoir that gives up ECF to plasma...

  • Lymphoscintigraphy in Lymphedema of the Lower Limbs Using 99mTc HSA. Nawaz, M. K.; Hamad, M. M.; Abdel-Dayem, H. M.; Sadek, S.; Eklof, B. G. H. // Angiology;Feb1992, Vol. 43 Issue 2, p147 

    Edema of the lower limbs is a difficult clinical problem. Edema could be due to stasis, obstruction of the lymphatic channels, or increased production of lymph beyond the drainage capacity of the lymphatic vessels. Sometimes It is difficult to differentiate among these varieties....

  • lecithin.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1311 

    A definition of the term "lecithin," which refers to a phospholipid that is part of cell membranes, is presented.

  • Phospholipids offer nutritional benefits.  // Wellness Foods Europe;Nov2011, Issue 3, p30 

    The article discusses the nutritional benefits of phospholipids, the functional ingredient of lecithin. The biochemistry of the most important phospholipids processed from lecithin, namely, phosphatidylcholine (LIPAMIN PC) phosphatidyserine (SERINAid PS) and glycerol-phospho-choline (GPC) is...

  • Crystallization of water in multilamellar vesicles. Lefèvre, Thierry; Toscani, Siro; Picquart, Michel; Dugué, Jérome // European Biophysics Journal;2002, Vol. 31 Issue 2, p126 

    The two-step crystallization of water in multilamellar vesicles (MLVs) of phosphatidylcholines has been investigated. The main crystallization occurs near –15 °C and involves bulk water. Contrary to unilamellar vesicles, a sub-zero phase transition is observed for MLVs at –40...

  • The influence of 1-β- D-arabinofuranosylcytosine on the metabolism of phosphatidylcholine in human leukemic HL 60 and Raji cells. Berkovic, D; Fleer, E A M; Breass, J; Pförtner, J; Schleyer, E; Hiddemann, W // Leukemia (08876924);Dec97, Vol. 11 Issue 12, p2079 

    We report that high-dose 1-β-D-arabinofuranosylcytosine (Ara-C) treatment leads to substantial changes of membrane lipid composition in human leukemic cell lines. HL 60 cells are at least 10- to 20-fold more sensitive to Ara-C than Raji cells. After 4 h incubation with 50 μM Ara-C, both...

  • Comparative Study of Different Silymarin Formulations: Formulation, Characterisation and In Vitro / In Vivo Evaluation. Abrol, Sonia; Trehan, Aman; Katare, Om Parkash // Current Drug Delivery;Mar2005, Vol. 2 Issue 1, p45 

    The aim of the present study was to study the synergistic hepatoprotective effect of silymarin with phospholipids when it is encaged in microspheres so as to passively target it to liver and to compare these silymarin formulations with silymarin solution. Various silymarin loaded lipid emulsions...

  • Phospholipid biosynthesis in mammalian cells. Vance, Jean E.; Vance, Dennis E. // Biochemistry & Cell Biology;Feb2004, Vol. 82 Issue 1, p113 

    Identification of the genes and gene products involved in the biosynthesis of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine has lagged behind that in many other fields because of difficulties encountered in purifying the respective proteins. Nevertheless, most of these...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics