Detection of urinary interleukin-8 in glomerular diseases

Wada, Takashi; Yokoyama, Hitoshi; Tomosugi, Naohisa; Hisada, Yukimasa; Ohta, Satoshi; Naito, Takero; Kobayashi, Ken-ichi; Mukaida, Naofumi; Matsushima, Kouji
August 1994
Kidney International;Aug1994, Vol. 46 Issue 2, p455
Academic Journal
Detection of urinary interleukin-8 in glomerular diseases. To clarify the mechanism of neutrophil infiltration in glomerulonephritis, both urinary and plasma levels of a potent neutrophil chemotactic cytokine, interleukin-8 (IL-8), were measured in 40 healthy volunteers and 96 patients with various renal diseases. The plasma IL-8 levels were less than 16 pg/ml. The urinary IL-8 levels were elevated in several renal diseases including IgA nephropathy (17 of 43), acute glomerulonephritis (4 of 6), lupus nephritis (11 of 15), purpura nephritis (2 of 4), membranoproliferative glomerulonephritis (1 of 1), and cryoglobulinemia (2 of 2), IL-8 was detected immunohistochemically in diseased glomeruli, suggesting its local production. Elevated urinary IL-8 levels during the acute phase or exacerbations were found to be decreased during spontaneous or steroid pulse therapy-induced convalescence in all patients examines. The urinary IL-8 levels were higher in patients with glomerular leukocyte infiltration than in those without infiltration. Collectively, local production of IL-8 in diseased glomeruli might be involved in the pathogenesis of the glomerular diseases and measurement of IL-8 in the urine might be useful for monitoring the glomerular diseases.


Related Articles

  • Polymorphonuclear leukocytes increase glomerular albumin permeability via hypohalous acid. Jing Zi Li; Sharma, Ram; Dileepan, Kottarappat N.; Savin, Virginia J. // Kidney International;Oct1994, Vol. 46 Issue 4, p1025 

    Acute glomerulonephritis is characterized by the presence of neutrophils within glomeruli and the generation of reactive oxygen species (ROS) by activated polymorphonuclear Ieukocytes (PMNs). Hydrogen peroxide (H2O2) and other ROS including hypohalous acids have been implicated in PMN mediated...

  • Lipoxins, leukocyte recruitment and the resolution phase of acute glomerulonephritis. O'Meara, Yvonne M.; Brady, Hugh R. // Kidney International Supplement;Mar1997, Issue 58, pS-56 

    The resolution phase of inflammation is being increasingly recognized as a dynamic multifaceted process whose components may be amenable to pharmacological manipulation for therapeutic gain. Here, we review evidence that the lipoxins (LX), a family, of lipoxygenase-derived eicosanoids generated...

  • Anti-inflammatory lymphokine mRNA expression in antibody-induced glomerulonephritis. Lakkis, Fadi G.; Baddoura, Fady K.; Cruet, Eddie N.; Parekh, Kannan R.; Fukunaga, Megumu; Munger, Karen A. // Kidney International;Jan1996, Vol. 49 Issue 1, p117 

    T helper subset 2 (Th2) lymphocytes produce interleukin 4 (IL-4) and IL-10, which exert anti-inflammatory actions on monocytes and macrophages. Th1 lymphocytes, on the other hand, secrete interferon-γ (IFNγ) which promotes tissue inflammation. The functional dichotomy between Th1 and Th2...

  • Incidence of biopsy-proven glomerulonephritis. Ole Wirta; Jukka Mustonen; Heikki Helin; Amos Pasternack // Nephrology Dialysis Transplantation;Jan2008, Vol. 23 Issue 1, p193 

    Background. The reported biopsy-proven glomerulonephritis incidence varies according to population characteristics, the unknown true glomerulonephritis incidence and biopsy rate. Reported glomerulonephritis incidence should be evaluated against the biopsy rate. Methods. We report here the...

  • Natural history and treatment of primary proliferative glomerulonephritis: A review. Glassock, Richard J. // Kidney International Supplement;Dec1985, Issue 17, pS136 

    Discussed the aspects of the natural history and treatment of primary proliferative glomerulonephritis. Classification of proliferative glomerulonephritis; Association of severe hypertension with the development of focal and segmental glomerulosclerosis and diffuse interstitial fibrosis;...

  • Cytokine-activated human mesangial cells generate the neutrophil chemoattractant, interleukin 8. Brown, Zarin; Strieter, Robert M.; Chensue, Stephen W.; Ceska, Miroslav; Lindley, Ivan; Neild, Guy H.; Kunkel, Steven L.; Westwick, John // Kidney International;Jul1991, Vol. 40 Issue 1, p86 

    Human mesangial cells (MC) in culture, when stimulated by interleukin 1α(IL-1α) or tumour necrosis factor (TNFα), but not with lipopolysaccharide (LPS), express interleukin 8 (IL-8) mRNA, and both cell associated and extracellular IL-8. Dexamethasone treatment of mesangial cells reduced...

  • Complement membrane attack (MAC) in idiopathic IgA-glomerulonephritis. Rauterberg, Ernst W.; Lieberknecht, Hans-Michael; Wingen, Anne-Margret; Ritz, Eberhard // Kidney International;Mar1987, Vol. 31 Issue 3, p820 

    Antigens of the membrane attack complex of complement (MAC), such as C5, C6, C9 and MAC-related neoantigen(s), were demonstrated in the mesangium of 23 cases with IgA-glomerulonephritis (IgA-GN) and two cases with Henoch-Schönlein purpura nephritis (HSP). High specificity of the polyclonal...

  • Differential effects of steroids on leukocyte-mediated glomerulonephritis in the rabbit. Holdsworth, Stephen R.; Bellomo, Rinaldo // Kidney International;Aug1984, Vol. 26 Issue 2, p162 

    The effects of steroids on the development of injury in two models of experimental glomerulonephritis (GN), (one mediated by neutrophils, the other by macrophages) were compared. The neutrophil-associated lesion [initiated by heterologous antiglomerular basement membrane (GBM) antibody] was...

  • Importance of checking anti-glomerular basement membrane antibody status in patients with anti-neutrophil cytoplasmic antibody-positive vasculitis. Gallagher, J. L.; Sinha, S.; Reeve, R.; Kalra, P. A. // Postgraduate Medical Journal;Apr2008, Vol. 84 Issue 990, p220 

    The case is reported of a 68-year-old man with perinuclear anti-neutrophil cytoplasmic antibody (pANCA)- associated glomerulonephritis who developed antibodies to glomerular basement membrane (anti-GBM) resulting in end stage renal failure. His pANCA titre on admission was 1:1024 lgG and he was...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics