TITLE

C5b-9 increases albumin permeability of isolated glomeruli in vitro

AUTHOR(S)
Savin, Virginia J.; Johnson, Richard J.; Couser, William G.
PUB. DATE
August 1994
SOURCE
Kidney International;Aug1994, Vol. 46 Issue 2, p382
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Deposition of antibody and activation of the complement cascade are important in both naturally occurring glomerulonephritis and in experimental models including passive Heymann nephritis. We studied the effect of antibody and complement on albumin permeability of isolated glomeruli to determine that role of the terminal complement components (C5-C9) in mediating the proteinuria in nephritis. Isolated glomeruli were treated with anti-Fx1a (Heymann antibody) and then incubated them with pooled human serum, serum in which complement had been inactivated by heal, or serum deficient in C6 or C7. The albumin reflection coefficient (σalbumin) was calculated from the volumetric response of glomeruli to transcapillary oncotic gradients produced by albumin or high molecular weight neutral dextran (252 kD). Convectional permeability to albumin (Palbumin) was calculated as 1-σalbumin. Albumin permeability of control glomeruli was not different from 0. Albumin permeability was not altered by antibody alone but was increased to 0.65 ± 0.04 when antibody treated glomeruli were incubated for 10 minutes with pooled serum as a source of complement. Heat treatment of serum to inactivate complement prevented the increase in permeability. Incubation for 10 minutes with serum without antibody pretreatment caused a lesser increase in permeability of isolated glomeruli (0.18 ± 0.06). Serum deficient in either C6 or C7 did not cause an increase in albumin permeability of antibody pre-treated glomeruli, but incubation with a combination of these sera (now containing the complete cascade) increased permeability to the same extent as did pooled normal serum (0.58 ± 0.04). We conclude that activation of the terminal complement components is required for the increase in glomerular macromolecular permeability caused by anti-Fx1a and that terminal complement activation is sufficient to alter the permeability independent of complement hemodynamic events or contribution by circulating cells.
ACCESSION #
14889141

 

Related Articles

  • Abstracts Australasian Society of Nephrology.  // Kidney International;Nov1985, Vol. 28 Issue 5, p856 

    Presents several on nephrology. "Leucocyte-Independent Glomerular Injury," by N. W. Boyce and S. R. Holdsworth; Glomerular Proacoagulant Activity (PCA) in Exprimental Anti-Glomerular Basement Membrane Antibody-Induced Glomerulonephritis," by P. G. Tipping and S. R. Holdsworth; "IgA and IgG...

  • Complement membrane attack (MAC) in idiopathic IgA-glomerulonephritis. Rauterberg, Ernst W.; Lieberknecht, Hans-Michael; Wingen, Anne-Margret; Ritz, Eberhard // Kidney International;Mar1987, Vol. 31 Issue 3, p820 

    Antigens of the membrane attack complex of complement (MAC), such as C5, C6, C9 and MAC-related neoantigen(s), were demonstrated in the mesangium of 23 cases with IgA-glomerulonephritis (IgA-GN) and two cases with Henoch-Schönlein purpura nephritis (HSP). High specificity of the polyclonal...

  • Biosynthesis of C3 by human mesangial cells. Modulation by proinflammatory cytokines. Montinaro, Vincenzo; Serra, Leonardo; Perissutti, Sabrina; Ranieri, Elena; Tedesco, Francesco; Schena, Francesco Paolo // Kidney International;Mar1995, Vol. 47 Issue 3, p829 

    Deposits of complement (C) components are found in the glomeruli of patients with various glomerulonephritides without detectable immunoglobulins, thus suggesting a pathogenetic role of the locally produced proteins of this system. In the present study, we have examined human mesangial cells...

  • Société de Nephrologie, Paris, France, February 9, 1974.  // Kidney International;Jul1974, Vol. 6 Issue 1, p254 

    The article presents abstracts related to research work in the field of nephrology. Some of the abstracts are "Serum Immunoglobulins in Mesangial IgA Deposit Glomerulonephritis," by G. LaGrue, G. Hirbec, M. Fournel and L. Intrator; "Serum IgE in Glomerular Diseases," by G. LaGrue, J.P. Moretti,...

  • Shining a LAMP on pauci-immune focal segmental glomerulonephritis. Salama, Alan D.; Pusey, Charles D. // Kidney International;Jul2009, Vol. 76 Issue 1, p15 

    Anti-neutrophil cytoplasm antibody (ANCA)–associated vasculitis frequently presents with renal involvement manifested by a focal segmental necrotizing glomerulonephritis, which is typically pauci-immune. Although considerable insight has been gained regarding potential mechanisms of organ...

  • Shared idiotypes in mesangial deposits in IgA nephropathy are not disease-specific. Van Den Wall Bake, A. Warmold L.; Bruijn, Jan Anthonie; Accavitti, Mary Ann; Crowley-Nowick, Peggy A.; Schrohenloher, Ralph E.; Julian, Bruce a.; Jackson, Susan; Kubagawa, Hiromi; Cooper, Max D.; Daha, Mohamed R.; Mestecky, Jiri // Kidney International;Jul1993, Vol. 44 Issue 1, p65 

    The antigenic specificity of the mesangial IgA in IgA nephropathy (IgAN) remains unknown. Because shared antigenic specificities may be reflected in the usage of shared idiotypes, we prepared five monoclonal anti-idiotypic antibodies (MoAbs) specific for the mesangial IgA eluted from the kidney...

  • Fibrillary glomerulonephritis without immunoglobulin deposits in the kidney. Churg, Jacob; Venkataseshan, V. Surya // Kidney International;Oct1993, Vol. 44 Issue 4, p837 

    Three patients are presented, who by electron microscopy, showed prominent fibrillary deposits in the glomeruli, and in two, also around the tubules. By immunohistology these two cases had no immunoglobulins in either glomeruli or around the tubules. In the third case, which probably represents...

  • The glomerular peripolar cell. Alcorn, Daine; Ryan, Graeme B. // Kidney International Supplement;Jul1993, Issue 42, pS-35 

    The PPC is a distinctive granulated epithelial cell at the vascular pole of the glomerulus. It is present in a wide range of animal species. It is especially prominent in sheep, particularly the newborn lamb. To dale, it has been shown that its granules contain albumin, immunoglobulins,...

  • Glomerular deposits and hypoalbuminemia in acute post-streptococcal glomerulonephritis. West, Clark D.; McAdams, A. James // Pediatric Nephrology;1998, Vol. 12 Issue 6, p471 

    In a search for correlations between glomerular morphology and clinical manifestations in acute poststreptococcal glomerulonephritis, data for 40 biopsied patients were reviewed. A major correlation was observed between severe hypoalbuminemia and the absence of deposits on the paramesangial...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics